Epidemiology and Infection

Research Article

RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis

A. Haasea1, A. Meldera1, H. Smith-Vaughana1, D. Kempa1 and B. Curriea1

a1 Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia


Twenty-seven isolates of Burholderia pseudomallei (formerly Pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was clearly due to the original strain re-emerging unchanged, with identical RAPD patterns with all four primers.

(Accepted February 16 1995)