Epidemiology and Infection

Research Article

The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

T. G. Wreghitta1, V. J. Robinsona2, E. O Caula3, I. D. Paula3 and S. Gatleya4

a1 Clinical Microbiology and Public Health Laboratory, Level 6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW.

a2 Department of Pathology, University of Cambridge.

a3 Joint Regional Public Health Laboratory Service and District Virology Laboratory, Myrtle Road, Kingsdown, Bristol.

a4 Novo BioLabs Ltd., Downham House, Downham's Lane, Milton Road, Cambridge.

Abstract

A μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

(Accepted May 17 1988)

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