European Journal of Anaesthesiology



Original Article
(RD) Surgery

Effects of propofol on substance P-induced relaxation in isolated human omental arteries and veins


G. Bodelsson a1, K. Sandström a2, S. M. Wallerstedt a2, J. Hidestål a2, K. Törnebrandt a3 and M. Bodelsson a2c1
a1 Department of Obstetrics and Gynaecology, University Hospital, Malmö, Sweden
a2 Department of Anaesthesia and Intensive Care, University Hospital, Lund, Sweden
a3 Department of Anaesthesia and Intensive Care, Hospital of Helsingborg, Helsingborg, Sweden

Article author query
bodelsson g   [PubMed][Google Scholar] 
sandström k   [PubMed][Google Scholar] 
wallerstedt sm   [PubMed][Google Scholar] 
hidestål j   [PubMed][Google Scholar] 
törnebrandt k   [PubMed][Google Scholar] 
bodelsson m   [PubMed][Google Scholar] 

Abstract

To elucidate if an effect of propofol on endothelium-dependent relaxation could contribute to propofol-induced vasodilation, smooth muscle relaxation of isolated human omental artery and vein segments precontracted by endothelin-1 were measured. Substance P induced a concentration-dependent relaxation (mean ±SEM) in both artery (63 ± 8.4% of precontraction, n=9) and vein (60 ± 11%, n=7). The relaxation was enhanced by 10−6 M propofol (artery, 72 a 9.5%, n=9; vein, 81 ± 12%, n=7) but not affected by 10−7, 10−5 and 10−4 M propofol. In the presence of Nω-nitro-L-arginine methyl ester (nitric oxide synthase inhibitor), 10−6 M propofol still enhanced the substance P-induced relaxation in arteries but not veins, whereas 10−4 M propofol inhibited the relaxation in both arteries (rightward shift of the concentration-response curve) and veins (28 ± 7.5%, n=8). In the presence of potassium chloride (to prevent hyperpolarization), the enhancement of substance P-induced relaxation by 10−6 M propofol was abolished in both arteries and veins whereas 10−5 and 10−4 M propofol reduced the relaxation in arteries (38 ± 13% at 10−5 M, n=6; 30 ± 11% at 10−4 M, n=6) but not in veins. These results demonstrate that propofol, at lower, clinically relevant concentrations, promotes endothelium-dependent relaxation mediated via hyperpolarization in human omental arteries and via both nitric oxide and hyperpolarization in human omental veins.

(Published Online August 16 2006)
(Accepted June 2000)


Key Words: anaesthetics; intravenous; propofol; tachykinins; substance P; arteries; omental; veins; omental; endothelium; vascular.

Correspondence:
c1 Correspondence: Mikael Bodelsson, Department of Anaesthesia and Intensive Care, University Hospital, SE-221 85 Lund, Sweden