a1 Bacteriologist-in-charge, Serum Department, Lister Institute of Preventive Medicine.
Bile salt media have been in use since 1900, and during the seven years which have elapsed it has been shown that B. typhosus, B. enteritidis (Gaertner), B. coli communis and similar organisms grow on these media just as well as on any other nutrient media. At 37°C. the growth of most of the organisms of the air and soil is inhibited by bile salt media.
These media can therefore be used with confidence for the isolation of B. typhosus and other intestinal organisms.