Visual Neuroscience

Research Articles

Glutamate immunoreactivity in the cat retina: A quantitative study

Ljubomir Jojicha1 and Roberta G. Pourchoa1

a1 Department of Anatomy and Cell Biology, Wayne State University, Detroit

Abstract

Immunocytochemical methods were used to visualize glutamate immunoreactivity in the cat retina and to compare its localization with that of aspartate, GABA, and glycine. The cellular and subcellular distribution of glutamate was analyzed at the light-microscopic level by optical densitometry and at the electron-microscopic level by immunogold quantification. The findings were consistent with the proposed role for glutamate as the neurotransmitter of photoreceptors and bipolar cells as particularly high concentrations of staining were found in synaptic terminals of these cells. Ganglion cells were also consistently stained. Aspartate was totally colocalized with glutamate in neuronal cell bodies but the synaptic levels of aspartate were much lower than for glutamate. In addition to the staining of photoreceptor, bipolar, and ganglion cells, glutamate immunoreactivity was also observed in approximately 60% of the amacrine cells. These cells exhibited colocalization with either GABA or glycine. The elevated levels of Glu in amacrine cells may reflect its role as a transmitter precursor in GABAergic cells and as an energy source for mitochondria in glycinergic cells.

(Received September 12 1994)

(Accepted June 12 1995)

Footnotes

Reprint requests to: Roberta G. Pourcho, Department of Anatomy and Cell Biology, Wayne State University, 540 E. Canfield, Detroit, MI 48201, USA.