Visual Neuroscience


Localization of GABAA receptors in the rat retina

Ursula Greferatha1, Frank Müllera1, Heinz Wässlea1, Brenda Shiversa2 and Peter Seeburga2

a1 Max-Planck-Institut für Hirnforschung, Neuroanatomische Abteilung, Deutschordenstrasse 46, 6000 Frankfurt 71, Germany

a2 Laboratorium für Molekulare Neuroendokrinologie, ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, 6900 Heidelberg, Germany


Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian retina. The present paper describes the localization of GABAA receptors in the rat retina as revealed by in situ hybridization and immunocytochemistry.

In situ hybridization with probes against various a subunits revealed a marked differential expression pattern. The αl subunit gene is expressed mainly in the bipolar and horizontal cell layer, the α2 gene in the amacrine and ganglion cell layer, and the α4 gene in a subpopulation of amacrine cells. β subunit mRNA is present diffusely throughout the entire inner nuclear layer and in the ganglion cell layer.

The monoclonal antibody bd 17 (against β2/β3 subunits) stained subpopulations of GABAergic and glycinergic amacrine cells as well as some ganglion cells and bipolar cells. Immunoreactivity was not restricted to synaptic input sites. In the outer plexiform layer bipolar cell dendrites were immunoreactive; in the inner plexiform layer mainly amacrine and ganglion cell processes were labeled, and bipolar cell axons appeared unstained. The results demonstrate a strong heterogeneity of GABAA receptors in the retina.

(Received July 27 1992)

(Accepted October 19 1992)


p1 Present address of Frank Müller: Institut für biologische Infor-mationsverarbeitung, Forschungszentrum Jülich, Postfach 1913, 5170 Julich, Germany.


Reprint requests to: Ursula Greferath, Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-6000 Frankfurt 71, Germany.