Parasitology



Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species


J. CABLE a1c1, P. D. HARRIS a2, J. W. LEWIS a3 and J. M. BEHNKE a2
a1 School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
a2 School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
a3 School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, UK

Article author query
cable j   [PubMed][Google Scholar] 
harris pd   [PubMed][Google Scholar] 
lewis jw   [PubMed][Google Scholar] 
behnke jm   [PubMed][Google Scholar] 

Abstract

The gastro-intestinal (GI) nematode Heligmosomoides polygyrus is an important experimental model in laboratory mice and a well-studied parasite of wood mice in the field. Despite an extensive literature, the taxonomy of this parasite in different hosts is confused, and it is unclear whether laboratory and field systems represent the same or different Operational Taxonomic Units (OTUs). Molecular analyses reveal high sequence divergence between H. p. bakeri (laboratory) and H. p. polygyrus (field); 3% difference in the ribosomal DNA Internal Transcribed Spacers (ITS) and 8·6% variation in the more rapidly evolving mitochondrial cytochrome c oxidase I (COI) gene. The COI sequence of U.K. H. p. polygyrus is more similar to H. glareoli from voles than to H. p. bakeri, while a single isolate of H. p. polygyrus from Guernsey confirms the extent of genetic variation between H. p. polygyrus populations. Analysis of molecular variance demonstrated that mtCOI sequence variation is associated primarily with groups with distinct ITS2 sequences, and with host identity, but is not partitioned significantly with a single combined taxon H. polygyrus incorporating European and North American isolates. We conclude therefore that the laboratory OTU should be raised to the level of a distinct species, as H. bakeri from the laboratory mouse Mus musculus, and we reject the hypothesis that H. bakeri has diverged from H. polygyrus in the recent past following introduction into America. However, we are unable to reject the hypothesis that H. polygyrus and H. bakeri are sister taxa, and it may be that H. polygyrus is polyphyletic or paraphyletic.

(Received July 23 2005)
(Revised November 9 2005)
(Revised January 16 2006)
(Accepted January 16 2006)
(Published Online March 15 2006)


Key Words: heligmosomatid; Apodemus sylvaticus; GI nematodes.

Correspondence:
c1 School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK. Tel: 0044 292 0876022. Fax: 0044 292 0874305. E-mail: cablej@cardiff.ac.uk


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