Hostname: page-component-8448b6f56d-sxzjt Total loading time: 0 Render date: 2024-04-23T12:42:40.463Z Has data issue: false hasContentIssue false
  • English
  • Français

Confocal Microscopy, Computer Modeling, and Quantification of Glomerular Vascular Corrosion Casts

Published online by Cambridge University Press:  16 May 2006

Roger C. Wagner ,
Kirk Czymmek  and
Fred E. Hossler
Roger C. Wagner
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Kirk Czymmek
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA
Fred E. Hossler
Affiliation:
Department of Anatomy and Cell Biology, J.H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA
Get access

Abstract

Corrosion-casted capillary systems of the kidney glomerulus were imaged with confocal microscopy because of the fluorescence properties of the casting plastic. Acquisition of a z-series through the glomerular capillaries provided three-dimensional data sets from which surface-rendered models were generated. These models could be rotated and viewed from any angle and also contained quantitative information allowing cast surface area and volume measurements to be calculated. The computer-generated models were also skeletonized to form a one-voxel-thick skeleton of the original model. The skeleton exhibited the three-dimensional topology and network of the capillary bed, and interior capillary relations could also be viewed. Quantitative information such as the total capillary length and number of capillary intersects was calculated from the skeletonized model. Extending this method to noncorroded kidney specimens revealed not only the casted vessels but also cellular features of the adjacent tissues surrounding the capillaries.

Keywords

kidneyglomeruluscorrosion castsconfocal microscopycomputer modelingskeletonization
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 12 , Issue 3 , June 2006 , pp. 262 - 268
Copyright
© 2006 Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aharinejad, S.H. & Lametschwandtner, A. (Eds.). (1992). Microvascular corrosion casting scanning electron microscopy: Techniques and applications. Wein: Springer-Verlag.
Bosan, S., Kareco, T., Ruehlmann, D., Chen, K., & Walley, K. (2003). Three-dimensional capillary geometry in gut tissue. Microsc Res Tech 61, 428–437.Google Scholar
Boyer, C. (1956). The vascular pattern of the kidney glomerulus as revealed by plastic reconstruction from serial sections. Anat Rec 125, 433–441.Google Scholar
Castenholz, A. (1995). Examination of injected specimens by confocal laser scanning microscopy and scanning electron microscopy. Scanning Microsc 9, 1245–1254.Google Scholar
Czymmek, K., Wagner, R., Hossler, F.E., & Kao, R. (2001). Imaging and volumetric quantitation of vascular corrosion casts with laser scanning confocal microscopy. Microsc Microanal 6, 562.Google Scholar
Hossler, F. (1992). Some quantitative applications of vascular corrosion casting. Electron Microsc Soc Am Bull 22, 738–739.Google Scholar
Hossler, F. & Douglas, J.E. (2001). Vascular corrosion casting: Review of advantages and limitations in the application of some simple quantitative methods. Microsc Microanal 7, 253–264.Google Scholar
Hossler, F., Douglas, J., & Douglas, L. (1986). Anatomy and morphometry of myocardial capillaries studied with vascular corrosion casting and scanning electron microscopy: A method for rat heart. Scanning Electron Microsc 4, 1469–1475.Google Scholar
Hossler, F., Douglas, J., Verghese, A., & Neal, L. (1991). Microvascular architecture of the elastase emphysemic hamster lung. J Electron Microsc Tech 19, 406–418.Google Scholar
Kaczmarek, E. & Becker, R. (1997). Three-dimensional modeling of renal glomerular capillary networks. Anal Quant Cytol Histol 19, 93–101.Google Scholar
Kratky, R., Zeindler, C., Lo, D., & Roach, M. (1989). Quantitative measurement from vascular corrosion casts. Scanning Microsc 3, 937–943.Google Scholar
Minnich, B. & Lametschwandtner, A. (2000). Length measurements in microvascular corrosion casting: Two dimensional versus three dimensional morphometry. Scanning 22, 173–177.Google Scholar
Minnich, B., Leeb, H., Bernroider, E., & Lametschwandtner, A. (1999). Three-dimensional morphometry in scanning electron microscopy: A technique for accurate dimensional and angular measurements of microstructures using stereopair digitized images and digital analysis. J Microsc 195, 23–33.Google Scholar
Murakami, T. (1972). Vascular arrangement of the rat renal glomerulus: A scanning electron microscopic study of corrosion casts. Arch Histol Jpn 34, 87–107.Google Scholar
Nyengard, J. & Marcussen, N. (1993). The number of glomerular capillaries estimated by an unbiased and efficient stereological method. J Microsc 171, 27–37.Google Scholar
Palagyi, K., Sorantin, E., Halami, C., & Kuba, A. (1999). 3D thinning and its applications to medical image processing. TASK Q 3, 397–408.Google Scholar
Rodriguez-Baeza, A., Reina-De La Torre, F., Ortega-Sanchez, M., & Sahuquillo-Barris, J. (1998). Perivascular structures in corrosion casts of the central nervous system: A confocal laser and scanning electron microscope study. Anat Rec 252, 176–184.Google Scholar
Shea, S. (1979). Glomerular hemodynamics and vascular structure: The pattern and dimensions of a single rat glomerular capillary network reconstructed from ultrathin sections. Microvasc Res 36, 129–143.Google Scholar
Weiger, T., Lametschwandtner, A., & Stockmayer, P. (1986). Technical parameters of plastics (Mercox CL-2B and various methacrylates) using scanning electron microscopy of vascular casts. Scanning Electron Microsc 1, 243–252.Google Scholar
Wagner, R., Czymmek, K., & Hossler, F. (2005). Confocal microscopy, modeling and quantitation of corroded and noncorroded casts of microvascular systems. Microsc Microanal 11, 1208–1209.Google Scholar
Yim, P., Summers, R., & Choyke, P. (2000). Gray-scale skeletonization of small vessels in magnetic resonance angiograms. Trans Med Imag 19, 568–576.Google Scholar