Visual Neuroscience



Retinal bipolar cell types differ in their inventory of ion channels


ELENA  IVANOVA  a1 and FRANK  MÜLLER  a1 c1
a1 Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Jülich, Jülich, Germany

Article author query
ivanova e   [Google Scholar] 
muller f   [Google Scholar] 
 

Abstract

Bipolar cells were recorded in rat retinal slices to study the distribution of hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels. Patch-clamp whole cell measurements were combined with intracellular filling and recorded cells were morphologically identified. HCN channel isoforms HCN1-4 are differentially expressed in bipolar cells. Each bipolar cell type has a characteristic inventory of HCN channels. The combination of HCN channel currents and other voltage-gated currents can be used as a kind of “finger print” to electrophysiologically identify and classify bipolar cell types. Using this approach of combined electrophysiological and morphological classification we could identify a new ON-cone bipolar cell type.

(Received December 14 2005)
(Accepted January 20 2006)


Key Words: HCN channels; Ih; retina; bipolar cell; electrophysiology.

Correspondence:
c1 Address correspondence and reprint requests to: Dr. Frank Müller, Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Jülich, Leo-Brandt-Strasse, D-52425 Jülich, Germany. E-mail: f.mueller@fz-juelich.de
p1 Current address: Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany