Hostname: page-component-7c8c6479df-r7xzm Total loading time: 0 Render date: 2024-03-29T13:54:03.940Z Has data issue: false hasContentIssue false

RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups

Published online by Cambridge University Press:  01 February 2000

R. G. ROBERTS
Affiliation:
USDA, ARS, Tree Fruit Research Laboratory, 1104 N. Western Ave, Wenatchee, WA 98801, USA
S. T. REYMOND
Affiliation:
USDA, ARS, Tree Fruit Research Laboratory, 1104 N. Western Ave, Wenatchee, WA 98801, USA
B. ANDERSEN
Affiliation:
Department of Biotechnology, Bldg 221, Technical University of Denmark, DK-2800, Lyngby, Denmark
Get access

Abstract

Accurate identification of small-spored Alternaria spp. is challenging because of morphological plasticity under non-standard conditions and the common misapplication of the name A. alternata to a variety of morphologically distinct taxa. A system used by some authors of naming phytotoxigenic alternarias as pathotypes of A. alternata has further clouded the meaning and usefulness of this specific epithet. Two hundred and sixty isolates of small-spored Alternaria, primarily from fruit substrates, were segregated into morphological groups and then subjected to RAPD-PCR analysis using total genomic DNA and three different primers. When cultured under defined conditions, the pattern of sporulation observed at 50 × magnification was predictive of genetic relatedness as determined by cluster analysis of RAPD fragment patterns. In RAPD analyses, morphological groups or species were resolved as distinct branches of the dendrogram: Alternaria gaisen (= A. kikuchiana, A. alternata Japanese pear pathotype, group 2), A. longipes (= A. alternata tobacco pathotype, group 5), the ‘tenuissima’ group (group 5), the arborescens group (group 3) and the ‘infectoria’ group (group 6). Isolates in groups for which there were only a few representatives clustered as a branch. Analysis of RAPD fragment patterns confirmed that when Alternaria isolates are cultured and observed under defined conditions, their phenotypic plasticity is minimized and valid taxonomic separations can be made upon morphological characteristics. We conclude that A. alternata, A. gaisen, A. longipes and other small-spored saprotrophic or perthotrophic taxa are recognizable as morphologically distinct taxa. We suggest that the ‘pathotype’ system of naming small-spored Alternaria taxa confers no predictive value relative to observable morphological and genetic characters, and should be abandoned.

Type
Research Article
Copyright
© The British Mycological Society 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)