Parasitology

Research Article

A putative Leishmania panamensis/Leishmania braziliensis hybrid is a causative agent of human cutaneous leishmaniasis in Nicaragua

A. A. Bellia1 p1, M. A. Milesa1 and J. M. Kellya1 p2

a1 Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK

Abstract

As part of a survey of human leishmaniasis in Nicaragua we examined phenotypic and genotypic characteristics of 40 Leishmania isolates. We identified 3 distinct parasites associated with cutaneous disease in this area; Leishmania panamensis (40% of cases), Leishmania braziliensis (33%), and a strain which exhibits the heterozygous isoenzyme and DNA fingerprinting patterns expected of a L. panamensis/L. braziliensis hybrid (27%). There was complete correlation between the isoenzyme and DNA data for each of the putative hybrids examined. All of the ‘hybrids’ were obtained from foci in the northern region of the country where L. panamensis and L. braziliensis occur sympatrically. These observations provide strong evidence for sexual reproduction in New World Leishmania populations and suggest that it is of taxonomic and epidemiological significance.

(Received January 27 1994)

(Revised March 11 1994)

(Accepted March 11 1994)

Correspondence:

p1 Present address: Departamento de Parasitologia, Centro Nacional de Diagnostico y Referencia, Apartado 2900, Managua, Nicaragua

p2 Reprint requests to Dr J. M. Kelly.

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