Parasitology

Research Article

An artificial feeding technique for Glossina

S. K. Molooa1

a1 East African Trypanosomiasis Research Organization, Tororo, Uganda

An apparatus for the artificial feeding of tsetse has been described. Fresh defibrinated blood at 36 °C is circulated with a peristaltic pump through feeding capsules. The efficiency of the method has been compared with that of the Cockings' method and with the live host feeding. The flies exposed to feeding on this apparatus showed significantly higher feeding frequency than those allowed to feed on the Cockings' apparatus. Compared with the live host feeding, the feeding frequency for circulating blood method was slightly lower but the difference was not significant. Once the fly is induced to feed, it would in most cases complete engorgement unless disturbed. The rate of crop emptying in flies fed by the Cockings' method was abnormally slower as compared to those fed on the circulating blood. The present study indicates that for the successful artificial feeding of tsetse, it is essential to circulate the blood and regulate the temperature of the medium and the feeding surface to approximately 36 °C.

I wish to thank Mr S. B. Kutuza for technical help, and the Director of E.A.T.R.O. for permission to publish this work.

(Received April 19 1971)

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