Parasitology

Research Article

Coproantigen detection in rats experimentally infected with Strongyloides ratti

C. Nageswarana1, P. S. Craiga2* and E. Devaneya3

a1 Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA

a2 Department of Biological Sciences, University of Salford, Salford M5 4WT

a3 Department of Veterinary Parasitology, University of Glasgow, Glasgow G61 1QH

Abstract

The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens. The first detection of coproantigen coincided with patency and the coproantigen level remained elevated even when the parasite count was low. Following challenge infection, when examination of stool for larvae or ova was negative, coproantigen was detected by anti-adult somatic capture ELISA. Despite the use of antisera raised against crude worm extracts, the test was specific showing only low cross-reactivity with Necator americanus and Syphacia muris.

(Received May 06 1993)

(Revised August 16 1993)

(Accepted August 16 1993)

Footnotes

* Reprint requests to: Professor P. S. Craig, Department of Biological Sciences, University of Salford, Salford M5 4WT.

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