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Trypanosoma (Nannomonas) congolense: molecular characterization of a new genotype from Tsavo, Kenya

Published online by Cambridge University Press:  06 April 2009

P. A. O. Majiwa ,
M. Maina ,
J. N. Waitumbi ,
S. Mihok  and
E. Zweygarth
P. A. O. Majiwa
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
M. Maina
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
J. N. Waitumbi
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
S. Mihok
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
E. Zweygarth
Affiliation:
Kenya Trypanosomiasis Research Institute (KETRI), P.O. Box 362, Kikuyu, Kenya and Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Berlin, Germany
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Summary

Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle and nuclear DNA sequences, and randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns have been used here to demonstrate the existence of another type of T. (N.) congolense that is genotypically distinct from those that have so far been characterized at the molecular level. A highly repetitive, tandemly arranged DNA sequence and oligonucleotide primers, for use in polymerase chain reaction (PCR) amplification are described, which can be used for specific identification of the trypanosome and its distinction from others within the Nannomonas subgenus.

Keywords

Trypanosoma congolensegenotypesDNA probePCRRFLPRAPD.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 151 - 162
Copyright
Copyright © Cambridge University Press 1993

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