Neuron Glia Biology



Articles

Electric field-induced astrocyte alignment directs neurite outgrowth


JOHN K. ALEXANDER a1, BABETTE FUSS a1 and RAYMOND J. COLELLO a1c1
a1 Department of Anatomy and Neurobiology, Medical College of Virginia, Virginia Commonwealth University, Richmond, USA

Article author query
alexander jk   [PubMed][Google Scholar] 
fuss b   [PubMed][Google Scholar] 
colello rj   [PubMed][Google Scholar] 

Abstract

The extension and directionality of neurite outgrowth are key to achieving successful target connections during both CNS development and during the re-establishment of connections lost after neural trauma. The degree of axonal elongation depends, in large part, on the spatial arrangement of astrocytic processes rich in growth-promoting proteins. Because astrocytes in culture align their processes on exposure to an electrical field of physiological strength, we sought to determine the extent to which aligned astrocytes affect neurite outgrowth. To this end, dorsal root ganglia cells were seeded onto cultured rat astrocytes that were pre-aligned by exposure to an electric field of physiological strength (500 mV mm−1). Using confocal microscopy and digital image analysis, we found that neurite outgrowth at 24 hours and at 48 hours is enhanced significantly and directed consistently along the aligned astrocyte processes. Moreover, this directed neurite outgrowth is maintained when grown on fixed, aligned astrocytes. Collectively, these results indicate that endogenous electric fields present within the developing CNS might act to align astrocyte processes, which can promote and direct neurite growth. Furthermore, these results demonstrate a simple method to produce an aligned cellular substrate, which might be used to direct regenerating neurites.

(Published Online January 23 2006)
(January 23 2006)


Key Words: Astrocytes; neurite; DRG; alignment; electric.

Correspondence:
c1 Raymond J. Colello, Department of Anatomy and Neurobiology, Medical College of Virginia, Virginia Commonwealth University, PO Box 980709, Richmond, VA 23298-0709, USA. phone: +01 804 828 2262; fax: +01 804 827 0698. email: rcolello@hsc.vcu.edu