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A female-specific cDNA sequence of Schistosoma mansoni encoding a mucin-like protein that is expressed in the epithelial cells of the reproductive duct*

Published online by Cambridge University Press:  06 April 2009

M. Menrath ,
A. Michel  and
W. Kunz
M. Menrath
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
A. Michel
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
W. Kunz
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
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Summary

Seven cDN A clones of Schistosoma mansoni containing the C-terminal part of the deduced sequence of a mucin-like protein have been identified. The protein contains 28% threonines, 20% serines, and has a pi of 3middot;4. On Northern blots of RNA of adult worms, the cDNA clones detect 2 transcripts of 1middot;65 and 4middot;2 kb which are expressed only in female worms. The tissue of gene expression, as revealed by in situ hybridization, is the epithelium surrounding the female reproduction duct proximal to its entrance into the ootype. Accumulation of N-glycosylation sites suggests that the protein, like other mucins, might form a protective layer, coating the lining of the duct. Regarding its acidic pi, we hypothesize a role in preventing premature egg-shell formation. This is the first female-specifically transcribed sequence, hitherto known in S. mansonithat is not expressed in the vitellaria.

Keywords

Schistosoma mansonimucin-like proteinfemale-specific cDNA sequence
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 477 - 483
Copyright
Copyright © Cambridge University Press 1995

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