Parasitology

Research Article

A cloned kinetoplast DNA mini-circle fragment from a Leishmania spp. specific for post-kala-azar dermal leishmaniasis strains

S. Das Guptaa1, D. K. Ghosha1 and H. K. Majumdera1*

a1 Leishmania Group, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India

Abstract

DNA–DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.

(Received May 01 1990)

(Revised August 30 1990)

(Accepted September 13 1990)

Footnotes

* Reprint requests to Dr H. K. Majumder.

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