Genetical Research

A recombinational portrait of the Drosophila pseudoobscura genome

a1 Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
a2 DCMB Group/Biology Department, Box 91000, Duke University, Durham, NC 27708, USA

Article author query
ortiz-barrientos d   [PubMed][Google Scholar] 
chang as   [PubMed][Google Scholar] 
noor ma   [PubMed][Google Scholar] 


Drosophila pseudoobscura has been intensively studied by evolutionary biologists for over 70 years. The recent publication of the genome sequence not only permits studies of comparative genomics with other dipterans but also opens the door to identifying genes associated with adaptive traits or speciation or testing for the signature of natural selection across the genome. Information on regional rates of recombination, localization of inversion breakpoints distinguishing it from its sibling species D. persimilis, and known polymorphic markers may be useful in such studies. Here, we present a molecular linkage map of four of the five major chromosome arms of D. pseudoobscura. In doing so, we order and orient several sequence contig groups, localize the inversion breakpoints on chromosome 2 to intervals of 200 kilobases, and identify one error in the published sequence assembly. Our results show that regional recombination rates in D. pseudoobscura are much higher than in D. melanogaster and significantly higher even than in D. persimilis. Furthermore, we detect a non-significant positive correlation between recombination rate and published DNA sequence variation. Finally, the online Appendix presents 200 primer sequence pairs for molecular markers that can be used for mapping of quantitative trait loci, of which 125 are known to be polymorphic within or between species.

(Published Online March 14 2006)
(Received September 9 2005)
(Revised November 2 2005)

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