Parasitology



The effect of chicory (Cichorium intybus) and sulla (Hedysarum coronarium) on larval development and mucosal cell responses of growing lambs challenged with Teladorsagia circumcincta


O. TZAMALOUKAS a1a2c1, S. ATHANASIADOU a1, I. KYRIAZAKIS a1a3, J. F. HUNTLEY a2 and F. JACKSON a2
a1 Animal Nutrition and Health Department, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
a2 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK
a3 Faculty of Veterinary Medicine, University of Thessaly, Karditsa, 43100, Greece

Article author query
tzamaloukas o   [PubMed][Google Scholar] 
athanasiadou s   [PubMed][Google Scholar] 
kyriazakis i   [PubMed][Google Scholar] 
huntley jf   [PubMed][Google Scholar] 
jackson f   [PubMed][Google Scholar] 

Abstract

The aim of this study was to investigate the effects of grazing different bioactive forages on acquired immunity against Teladorsagia circumcinta infection. The development of immunity was assessed by following the response of trickle-infected lambs grazing chicory (Cichorium intybus; IC), sulla (Hedysarum coronarium; IS) or grass/clover (Lolium perenne/Trifolium repens; IGC), to a single challenge infection. Parasite-naive lambs, grazing grass/clover, were also challenged with the single infection dose providing the uninfected control (UGC) group. Trickle infection significantly reduced worm establishment, inhibited larval development and increased mucosal mast cell (MMC) and globule leucocyte (GL) cells. Grazing treatment (chicory, sulla or grass/clover) significantly affected adult worm (P<0·05), late-L4 (P<0·01) and mid-L4 (P<0·01) larval-stage recoveries of the trickle-infected lambs, with IGC group always carrying higher worm burdens than either IC or IS lambs. MMC and GL cells of trickle-infected lambs were positively correlated with the proportion of early-L4 worms recovered and negatively correlated with both the proportion of adult worms recovered and the total worm establishment, suggesting that the observed effects were due to an enhanced immune response. The results suggest elevated immune responses against T. circumcincta infections in growing lambs grazing on either sulla or chicory compared to those grazing on grass/clover, probably due to differences in forage nutritional values.

(Received May 25 2005)
(Revised August 9 2005)
(Revised August 11 2005)
(Revised September 15 2005)
(Accepted September 15 2005)
(December 7 2005)


Key Words: Teladorsagia circumcincta; sheep; immunity; Cichorium intybus; Hedysarum coronarium; gastrointestinal nematode.

Correspondence:
c1 Scottish Agricultural College, Sir Stephen Watson Building, Bush Estate, Penicuik, Midlothian EH26 0PH, UK. Tel: +44 131 535 3058. Fax: +44 131 535 3121. E-mail: Ouranios.Tzamaloukas@sac.ac.uk


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