Hostname: page-component-7c8c6479df-94d59 Total loading time: 0 Render date: 2024-03-29T06:56:11.226Z Has data issue: false hasContentIssue false

An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Encephalitozoon cuniculi and its use in determination of infections in man

Published online by Cambridge University Press:  06 April 2009

Wafaa S. Hollister
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, London 8W7 2BB
Elizabeth U. Canning
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, London 8W7 2BB

Summary

An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Encephalitozoon cuniculi in man, using spores derived from tissue culture as antigen. Negligible cross-reactions were found with other microsporidia of vertebrate or of invertebrate origin and there was no cross-reaction with Toxoplasma gondii, using ELISA, immunoperoxidase or immunofluorescence staining. A high prevalence of antibodies to E. cuniculi was found in patients suffering from schistosomiasis, malaria and neurological and psychiatric disorders, but not in healthy individuals.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bergquist, N. R., Stintzing, G., Smedman, L., Waller, T. & Andersson, T. (1984). Diagnosis of encephalitozoonosis in man by serological tests. British Medical Journal 288, 902.Google Scholar
Bywater, J. E. C. & Kellett, B. S. (1979). Humoral immune response to natural infection with Encephalitozoon in rabbits. Journal of Pathology and Bacteriology 27, 329–33.Google Scholar
Canning, E. U. & Lom, J. (1986). The Microsporidia of Vertebrates. Pp. 289. New York and London: Academic Press.Google Scholar
Chalupsky, J., Vávra, J. & Bedrník, P. (1979). Encephalitozoonosis in laboratory animals-a serological survey. Folia Parasitologica 26, 18.Google ScholarPubMed
Cox, J. C. & Gallichio, H. A. (1977). An evaluation of immunofluorescence in the serological diagnosis of Nosema cuniculi infection. Research in Veterinary Science 22, 50–2.Google Scholar
Cox, J. C. & Gallichio, H. A. (1978). Serological and histological studies on adult rabbits with recent, naturally acquired encephalitozoonosis. Research in Veterinary Science 24, 260–1.CrossRefGoogle ScholarPubMed
Cox, J. C., Horsburgh, R. & Pye, D. (1981). Simple diagnostic test for antibodies to Encephalitozoon cuniculi based on enzyme immunoassay. Laboratory Animals 15, 41–3.Google Scholar
Cox, J. C. & Pye, D. (1975). Serodiagnosis of nosematosis by immunofluorescence using cell culture organisms. Laboratory Animals 9, 297304.Google Scholar
Cox, J. C. & Ross, J. (1980). A serological survey of Encephalitozoon cuniculi infection in the wild rabbit in England and Scotland. Research in Veterinary Science 28, 396.CrossRefGoogle Scholar
Irby, W. S., Huang, Y. S., Kawanishi, C. Y. & Brooks, W. M. (1986). Immunoblot analysis of exospore polypeptides from some entomophilic microsporidia. Journal of Protozoology 33, 1420.CrossRefGoogle Scholar
Matsubayashi, H., Koike, T., Mikata, I., Takei, H. & Hagiwara, S. (1959). A case of Encephalitozoon-like infection in man. Archives of Pathology 67, 181–7.Google ScholarPubMed
Niederkorn, J. Y., Shadduck, J. A. & Weidner, E. (1980). Antigenic cross reaction among different microsporidian spores as determined by immunofluorescence. Journal of Parasitology 66, 675–7.Google Scholar
Schmidt, E. C. & Shadduck, J. A. (1983). Murine encephalitozoonosis model for studying the host-parasite relationship of a chronic infection. Infection and Immunity 40, 936–42.Google Scholar
Singh, M., Kane, G. J., Mackinlay, L., Quaki, I., Yap, E. H., Ho, B. C., Ho, L. C. & Lim, K. C. (1982). Detection of antibodies to E. cuniculi (Protozoa, Microsporidia) in human and animal sera by the indirect fluorescent antibody technique. Southeast Asian Journal of Tropical Medicine and Public Health 13, 110–13.Google Scholar
Sprague, V. (1977). The zoological distribution of the microsporidia. In Systematics of the Microsporidia, Comparative Pathobiology, vol. 2 (ed. Bulla, L. A. and Cheng, T. C.), pp. 335385. New York and London: Plenum Press.Google Scholar
Stewart, C. G., Botha, W. S. & van Dellen, A. F. (1979). The prevalence of Encephalitozoon antibodies in dogs and an evaluation of the indirect fluorescent antibody test. Journal of the South African Veterinary Association 50, 169–72.Google Scholar
Voller, A., Bidwell, D. E. & Bartlett, A. (1979). The Enzyme Linked Immunosorbent Assay (ELISA). A Guide with Abstracts of Microplate Applications. Guernsey: Dynatech Europe.Google Scholar
Waller, T. (1975). Growth of Noasema cuniculi in established cell lines. Laboratory Animals 9, 61–8.Google Scholar
World Health Organization, Weekly Epidemiological Record (1983). Parasitic disease surveillance: antibody to Encephalitozoon cuniculi in man. No. 2–3 02 58, 3032.Google Scholar