Molecular typing of Escherichia coli O157[ratio]H7 (H−) isolates from cattle in Japan
A total of 77 Escherichia coli O157[ratio]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio]H7 infection in Japan.(Accepted December 2 1998)
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