Epidemiology and Infection



Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows


J. R. FITZGERALD a1a3, W. J. MEANEY a2, P. J. HARTIGAN a1, C. J. SMYTH a3 and V. KAPUR a4
a1 Department of Physiology, Trinity College, Dublin 2, Ireland
a2 Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
a3 Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin, Ireland
a4 Department of Veterinary Pathobiology, School of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA

Abstract

Sixty-three Staphylococcus aureus isolates recovered from bovine sources in the USA and the Republic of Ireland were characterized by multilocus enzyme electrophoresis (MLEE), ribotyping, and random amplified polymorphic DNA polymerase chain reaction (RAPD–PCR) typing at two separate laboratories. The S. aureus isolates were assigned by MLEE to 10 electrophoretic types (ETs) (Index of Discrimination, D=0·779). In contrast, the same isolates were assigned to 13 ribotypes (D=0·888), and to 12 RAPD types (D=0·898). A common clone, ET3, of worldwide distribution, was represented by six distinct combinations of ribotypes and RAPD types. S. aureus clones recovered from cows in Ireland were also associated with mastitis in dairy cows in the USA. These findings are consistent with the hypothesis that only a few specialized clones of S. aureus are responsible for the majority of cases of bovine mastitis, and that these clones have a broad geographic distribution.

(Accepted May 26 1997)


Correspondence:
Author for correspondence: J. Ross Fitzgerald, Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Republic of Ireland.


Metrics