Genetical Research



Distinguishing recombination and intragenic gene conversion by linkage disequilibrium patterns


THOMAS WIEHE a1c1, JOANNA MOUNTAIN a1p1, PETER PARHAM a2 and MONTGOMERY SLATKIN a1
a1 Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA
a2 Department of Structural Biology, Stanford University, Stanford, CA 94305, USA

Abstract

Deterministic theory suggests that reciprocal recombination and intragenic, interallelic conversion have different effects on the linkage disequilibrium between a pair of genetic markers. Under a model of reciprocal recombination, the decay rate of linkage disequilibrium depends on the distance between the two markers, while under conversion the decay rate is independent of this distance, provided that conversion tracts are short. A population genetic three-locus model provides a function Q of two-locus linkage disequilibria. Viewed as a random variable, Q is the basis for a test of the relative impact of conversion and recombination. This test requires haplotype frequency data of a sufficiently variable three-locus system. One of the few examples currently available is data from the Human Leukocyte Antigen (HLA) class I genes of three Amerindian populations. We find that conversion may have played a dominant role in shaping haplotype patterns over short stretches of DNA, whereas reciprocal recombination may have played a greater role over longer stretches of DNA. However, in order to draw firm conclusions more independent data are necessary.

(Received April 2 1999)
(Revised April 28 1999)


Correspondence:
c1 Corresponding author. Department of Molecular Genetics and Evolution, Max Planck Institute for Chemical Ecology, Tatzend- promenade 1, D-07745 Jena, Germany. Tel: +49-3641-64 3631. Fax: +49-3641-64 3668. e-mail: twiehe@ice.mpg.de.
p1 Current address: Dept. of Anthropological Sciences and Genetics, Stanford University, Stanford, CA 94305-2145, USA.


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