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Zygote

Table of Contents - 2005 - Volume 13, Issue 04  


Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer

Walt Yamazaki a1c1, Christina Ramires Ferreira a1, Simone Cristina Méo a1, Cláudia Lima Verde Leal a2, Flávio Vieira Meirelles a2 and Joaquim Mansano Garcia a1
a1 Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via, de Acesso Prof. Paulo Donato Castelane, s/n, 14884-900, jaboticabal, SP, Brazil.
a2 Departamento de Ciências Básicas, FZEA – USP, Av. Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil.

Article author query
yamazaki w   [PubMed][Google Scholar] 
ferreira cr   [PubMed][Google Scholar] 
meo sc   [PubMed][Google Scholar] 
leal cl   [PubMed][Google Scholar] 
meirelles fv   [PubMed][Google Scholar] 
garcia jm   [PubMed][Google Scholar] 

Abstract

As an important step in the nuclear transfer (NT) procedure, we evaluated the effect of three different treatments for oocyte activation on the in vitro and in vivo developmental capacity of bovine reconstructed embryos: (1) strontium, which has been successfully used in mice but not yet tested in cattle; (2) ionomycin and 6-dimethylaminopurine (6-DMAP), a standard treatment used in cattle; (3) ionomycin and strontium, in place of 6-DMAP. As regards NT blastocyst development, no difference was observed when strontium (20.1%) or ionomycin/6-DMAP (14.4%) were used. However, when 6-DMAP was substituted by strontium (3), the blastocyst rate (34.8%) was superior to that in the other activation groups (p <0.05). Results of in vivo development showed the possibility of pregnancies when NT embryos activated in strontium were transferred to recipient cows (16.6%). A live female calf was obtained when ionomycin/strontium were used, but it died 30 days after birth. Our findings show that strontium can be used as an activation agent in bovine cloning procedures and that activation with a combination of strontium and ionomycin increased the in vitro developmental capacity of reconstructed embryos. This is the first report of a calf produced by adult somatic cell NT in Latin America.

(Received February 14 2005)
(Accepted April 29 2005)


Key Words: Bovine; Cloning; Oocyte activation; Somatic cell; Strontium.

Correspondence:
c1 All correspondence to: W. Yamazaki: DMVPRA – FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castelane, s/n, 14884-900, Jaboticabal-SP, Brazil. Tel: +55 16 3209 2633. Fax: +55 16 3202 4275. e-mail: wyamazaki@yahoo.com

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