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The influence of dietary carbohydrates on experimental infection with Trichuris suis in pigs

Published online by Cambridge University Press:  13 September 2005

L. E. THOMSEN ,
S. PETKEVIČIUS ,
K. E. BACH KNUDSEN  and
A. ROEPSTORFF
L. E. THOMSEN
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, KVL, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark
S. PETKEVIČIUS
Affiliation:
Department of Infectious Diseases, Lithuania Veterinary Academy, LT-47181 Kaunas, Lithuania
K. E. BACH KNUDSEN
Affiliation:
Department of Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
A. ROEPSTORFF
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, KVL, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark
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Abstract

Two experiments (Exps 1 and 2) were carried out to study the effect of dietary carbohydrates on the establishment of Trichuris suis in pigs. Two experimental diets based on barley flour were used; Diet 1 was supplemented with non-fermentable carbohydrates from oat hull meal, while Diet 2 was supplemented with fermentable carbohydrates from sugar beet fibre and inulin. In Exp. 1, thirty-two pigs were allocated randomly into 4 groups. Two groups were fed Diet 1 and 2 groups were fed Diet 2. Pigs from one of each diet group were inoculated with 2000 infective T. suis eggs each and the other two groups were uninfected controls. All pigs were slaughtered 8 weeks post-inoculation (p.i.). In Exp. 2, twenty-four pigs were allocated randomly into 2 groups and fed Diet 1 or Diet 2, respectively. All the pigs were inoculated with 2000 infective T. suis eggs. Six pigs from each group were slaughtered 8 weeks p.i. and the remaining 6 pigs from each group were slaughtered 12 weeks p.i. Infections were followed by faecal egg counts and worm burdens were assessed at necropsy. Pigs fed Diet 2 had lower egg counts in both experiments; in Exp. 2 the difference was significant (P<0·05). No differences were found in worm burdens 8 weeks p.i. in both experiments, however, worms from pigs on Diet 2 were significantly shorter (P<0·0001). Pigs fed Diet 2 and slaughtered 12 weeks p.i. had significantly lower worm counts (P<0·01) compared to pigs fed Diet 1. The results indicate that fermentable carbohydrates do not affect the establishment of T. suis in naïve pigs, but result in earlier expulsion and reduced growth of the established worms. Thus, diets with highly fermentable carbohydrates may be used in the control of T. suis.

Keywords

Trichuris suispigsinulinsugar beet fibrenutrition
Type
Research Article
Information
Parasitology , Volume 132 , Issue 6 , June 2006 , pp. 857 - 865
Copyright
2005 Cambridge University Press

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References

REFERENCES

ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS ( 1990). Official Methods of Analyses of the Association of Official Analytical Chemists, 15th Edn. Association of Official Analytical Chemists, Inc., Virginia, USA.
Bach Knudsen, K. E. ( 1997). Carbohydrate and lignin contens of plant materials used in animal feeding. Animal Feed Science Technology 67, 319–338.CrossRefGoogle Scholar
Bach Knudsen, K. E. and Hessov, I. ( 1995). Recovery of inulin from Jerusalem artichoke (Helianthus tuberosus L.) in the small intestine of man. British Journal of Nutrition 74, 101–113.Google Scholar
Beer, R. J. S. ( 1973). Studies on the biology of the life-cycle of Trichuris suis Schrank, 1788. Parasitology 67, 253–262.CrossRefGoogle Scholar
Biehl, L. G. ( 1984). Internal parasitism of feeder pigs in Southern Illinois. Agri-Practice 5, 20–26.Google Scholar
Boisen, S. and Fernández, J. A. ( 1997). Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses. Animal Feed Science Technology 68, 277–286.CrossRefGoogle Scholar
Brunsgaard, G. ( 1998). Effects of cereal type and feed particle size on morphological characteristics, epithelial cell proliferation, and lectin binding patterns in the large intestine of pigs. Journal of Animal Science 76, 2787–2798.CrossRefGoogle Scholar
Burden, D. J. and Hammet, N. C. ( 1976). A comparison of the infectivity of Trichuris suis ova embryonated by four different methods. Veterinary Parasitology 2, 307–311.CrossRefGoogle Scholar
Burden, D. J., Hammet, N. C. and Brookes, P. A. ( 1987). Field observations on the longevity of Trichuris suis ova. Veterinary Record 121, 43.CrossRefGoogle Scholar
Carstensen, L., Vaarst, M. and Roepstorff, A. ( 2002). Helminth infections in Danish organic swine herds. Veterinary Parasitology 106, 253–264.CrossRefGoogle Scholar
Christensen, C. M., Barnes, E. H., Nansen, P., Roepstorff, A. and Slotved, H. C. ( 1995). Experimental Oesophagostomum dentatum infection in the pig: worm populations resulting from single infections with three doses of larvae. International Journal for Parasitology 25, 1491–1498.CrossRefGoogle Scholar
Coop, R. L. and Kyriazakis, I. ( 2001). Influence of host nutrition on the development and consequences of nematode parasitism in ruminants. Trends in Parasitology 17, 325–330.CrossRefGoogle Scholar
Crompton, D. W. T. ( 1999). How much human helminthiasis is there in the world? Journal of Parasitology 85, 397–403.Google Scholar
Cummings, J. H., Roberfroid, M. B., Andersson, H., Barth, C., Ferro-Luzzi, A., Ghoos, Y., Gibney, M., Hermonsen, K., James, W. P., Korver, O., Lairon, D., Pascal, G. and Voragen, A. G. ( 1997). A new look at dietary carbohydrate: chemistry, physiology and health. Paris Carbohydrate Group. European Journal of Clinical Nutrition 51, 417–423.CrossRefGoogle Scholar
Gibson, G. R., Beatty, E. R., Wang, X. and Cummings, J. H. ( 1995). Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975–982.CrossRefGoogle Scholar
Gibson, G. R. and Wang, X. ( 1994). Regulatory effects of bifidobacteria on the growth of other colonic bacteria. Journal of Applied Bacteriology 77, 412–420.CrossRefGoogle Scholar
Hill, D. E., Romanowski, R. D. and Urban, J. F., Jr. ( 1997). A Trichuris specific diagnostic antigen from culture fluids of Trichuris suis adult worms. Veterinary Parasitology 68, 91–102.CrossRefGoogle Scholar
Jensen, M. T., Cox, R. P. and Jensen, B. B. ( 1995). Microbial production of skatole in the hind gut of pigs given different diets and its relation to skatole deposition in backfat. Animal Science 61, 293–304.CrossRefGoogle Scholar
Jensen, T. K. and Svensmark, B. ( 1996). Trichuriasis hos udendørs slagtesvin. Veterinærinformation 2, 3–7.Google Scholar
Kelly-Quagliana, K. A., Nelson, P. D. and Buddington, R. K. ( 2003). Dietary oligofructose and inulin modulate immune functions in mice. Nutrition Research 23, 257–267.CrossRefGoogle Scholar
Mansfield, L. S. and Urban, J. F., Jr. ( 1996). The pathogenesis of necrotic proliferative colitis in swine is linked to whipworm induced suppression of mucosal immunity to resident bacteria. Veterinary Immunology and Immunopathology 50, 1–17.CrossRefGoogle Scholar
Nansen, P. and Roepstorff, A. ( 1999). Parasitic helminths of the pig: factors influencing transmission and infection levels. International Journal for Parasitology 29, 877–891.CrossRefGoogle Scholar
Pearce, G. P. ( 1999). Interactions between dietary fibre, endo-parasites and Lawsonia intracellularis bacteria in grower-finisher pigs. Veterinary Parasitology 87, 51–61.CrossRefGoogle Scholar
Pedersen, S. and Saeed, I. ( 2000). Experimental infection of pigs with three dose levels of Trichuris suis. Parasite 7, 275–281.CrossRefGoogle Scholar
Pedersen, S. and Saeed, I. ( 2001). Acquired immunity to Trichuris suis infection in pigs. Parasitology 123, 95–101.CrossRefGoogle Scholar
Petkevičius, S., Bach Knudsen, K. E., Murrell, K. D. and Wachmann, H. ( 2003). The effect of inulin and sugar beet fibre on Oesophagostomum dentatum infection in pigs. Parasitology 127, 61–68.CrossRefGoogle Scholar
Petkevičius, S., Bach Knudsen, K. E., Nansen, P. and Murrell, K. D. ( 2001). The effect of dietary carbohydrates with different digestibility on the populations of Oesophagostomum dentatum in the intestinal tract of pigs. Parasitology 123, 315–324.CrossRefGoogle Scholar
Petkevičius, S., Murrell, K. D., Bach Knudsen, K. E., Jørgensen, H., Roepstorff, A., Laue, A. and Wachmann, H. ( 2004). Effects of short-chain fatty acids and lactic acids on survival of Oesophagostomum dentatum in pigs. Veterinary Parasitology 122, 293–301.CrossRefGoogle Scholar
Petkevičius, S., Nansen, P., Bach Knudsen, K. E. and Skjøth, F. ( 1999). The effect of increasing levels of insoluble dietary fibre on the establishment and persistence of Oesophagostomum dentatum in pigs. Parasite 6, 17–26.CrossRefGoogle Scholar
Powers, K. G. ( 1959). Swine Whipworm in Wisconsin. Veterinary Medicine 54, 396–397.Google Scholar
Roediger, W. E. W. ( 1980). Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut 21, 793–798.CrossRefGoogle Scholar
Roepstorff, A. and Nansen, P. ( 1998). Epidemiology, Diagnosis and Control of Helminth Parasites of Swine. FAO Animal Health Manual. , FAO, Rome, Italy.Google Scholar
Schley, P. D. and Field, C. J. ( 2002). The immune-enhancing effects of dietary fibres and prebiotics. British Journal of Nutrition 87 (Suppl. 2), S221–S230.Google Scholar
Schürch, A. F., Lloyd, L. E. and Crampton, E. W. ( 1950). The use of chromic oxide as an index for determining the digestibility of a diet. The Journal of Nutrition 41, 629–636.CrossRefGoogle Scholar
Stephenson, L. S., Holland, C. V. and Cooper, E. S. ( 2000). The public health significance of Trichuris trichiura. Parasitology 121 (Suppl.), S73–S95.CrossRefGoogle Scholar
Stewart, T. B. and Hale, O. M. ( 1988). Losses to internal parasites in swine production. Journal of Animal Science 66, 1548–1554.CrossRefGoogle Scholar
Stoldt, W. ( 1952). Vorschlag zur Vereinheitlichung der Fettbestimmung in Lebensmitteln. Fette und Seifen 4, 206–207.CrossRefGoogle Scholar
Thamsborg, S. M., Roepstorff, A. and Larsen, M. ( 1999). Integrated and biological control of parasites in organic and conventional production systems. Veterinary Parasitology 84, 169–186.CrossRefGoogle Scholar
Wächtershäuser, A. and Stein, J. ( 2000). Rationale for the luminal provision of butyrate in intestinal diseases. European Journal of Nutrition 39, 164–171.CrossRefGoogle Scholar
Wang, X. and Gibson, G. R. ( 1993). Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75, 373–380.CrossRefGoogle Scholar