Parasitology



Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis


E.-G. LEE a1, Y.-A. BAE a1, Y.-T. JEONG a1, J.-Y. CHUNG a2, E.-Y. JE a1, S.-H. KIM a1, B.-K. NA a1, J.-W. JU a3, T.-S. KIM a3, L. MA a4, S.-Y. CHO a1 and Y. KONG a1c1
a1 Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
a2 PharmacoGenomics Research Center, Inje University, Busan 614-735, Korea
a3 Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
a4 Infectious Diseases Section, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

Article author query
lee eg   [PubMed][Google Scholar] 
bae ya   [PubMed][Google Scholar] 
jeong yt   [PubMed][Google Scholar] 
chung jy   [PubMed][Google Scholar] 
je ey   [PubMed][Google Scholar] 
kim sh   [PubMed][Google Scholar] 
na bk   [PubMed][Google Scholar] 
ju jw   [PubMed][Google Scholar] 
kim ts   [PubMed][Google Scholar] 
ma l   [PubMed][Google Scholar] 
cho sy   [PubMed][Google Scholar] 
kong y   [PubMed][Google Scholar] 

Abstract

Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of proteomic analyses. The protein was found to consist of 2 major components of 42–46 and 22–28 kDa, and shared 3 subunits of 14, 16 and 18 kDa. The 42–46 kDa component was determined to contain 3 additional subunits of 22, 28 and 38 kDa. These 6 subunits were shown to originate from either the 14 or 18 kDa precursor. We assessed the antibody reactivity of the native protein, its individual subunits and the recombinant 14 and 18 kDa proteins, and found that the 120 kDa protein, particularly 14 and 18 kDa subunits revealed high reliability for differentiation of active and mixed stage NC from chronic NC. The subunits of the 120 kDa protein complex identified herein represent some of the low-molecular weight glycoproteins which have been described in several previous studies. Recognizing and understanding the structural and immunological relationship of these proteins will facilitate the development of new serodiagnostic assays.

(Received March 21 2005)
(Revised May 7 2005)
(Accepted June 2 2005)


Key Words: Taenia solium neurocysticercosis; 120 kDa glycoprotein; MALDI-TOF MS; 14 kDa; 18 kDa; low-molecular weight glycoprotein; serodiagnostic antigen.

Correspondence:
c1 Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea. Tel: +82 31 299 6251. Fax: +82 31 299 6269. E-mail: ykong@med.skku.ac.kr


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