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Microwave Protocols for Paraffin Microtechnique and In Situ Localization in Plants

Published online by Cambridge University Press:  28 July 2005

Denise Schichnes ,
Jeff Nemson ,
Lorraine Sohlberg  and
Steven E. Ruzin
Denise Schichnes
Affiliation:
CNR Biological Imaging Facility, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720
Jeff Nemson
Affiliation:
CNR Biological Imaging Facility, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720
Lorraine Sohlberg
Affiliation:
CNR Biological Imaging Facility, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720
Steven E. Ruzin
Affiliation:
CNR Biological Imaging Facility, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720
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Abstract

We have developed a microwave protocol for a paraffin-embedding microtechnique of the shoot apical meristem of Zea mays and have successfully applied this protocol to other plant tissues. This protocol decreases the time required for all aspects of microtechnique tissue processing, including fixation (24 hr to 15 min), dehydration (73 hr to 10 min), and infiltration (96 hr to 3 hr). Additionally, the time required to adhere paraffin ribbons to gelatin-coated slides and for the Johanson's safranin O, fast green FCF staining protocol has been significantly decreased. Using this technique, the quality of tissue preservation and subsequent in situ localization of knotted mRNA was increased by using microwaves.

Keywords

in situmicrotechniquemicrowaveparaffinplantsstaining
Type
Research Article
Information
Microscopy and Microanalysis , Volume 4 , Issue 5 , October 1998 , pp. 491 - 496
Copyright
© 2005 Microscopy Society of America

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