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Differential infectivity and immunopathology in murine experimental infections by two natural clones belonging to the Trypanosoma cruzi I lineage

Published online by Cambridge University Press:  09 March 2005

E. GARZON
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
F. GENNA
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
M. F. BOSSENO
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
J. SIMONY-LA FONTAINE
Affiliation:
Département d'Anatomopathologie, CRLC Val d'Aurelle-Paul Lamarque, Parc Euromédecine, 34298, Montpellier cédex 5, France
M. RADAL
Affiliation:
Département d'Anatomopathologie, CRLC Val d'Aurelle-Paul Lamarque, Parc Euromédecine, 34298, Montpellier cédex 5, France
D. SERENO
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
F. MATHIEU-DAUDE
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
A. OUAISSI
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
S. F. BRENIÈRE
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France

Abstract

Immunopathology of Chagas' disease in Balb/c mice infected with 2 Trypanosoma cruzi clones, belonging to the T. cruzi I lineage and presenting different in vitro virulence (P/209 cl1>SO34 cl4) was compared. In the acute phase, evading mechanisms such as parasite-induced lymphocyte polyclonal activation and T cell immunosuppression were higher in mice infected with the clone giving a higher parasitaemia (P/209 cl1). A similar increase of non-specific isotypes was observed in both infections with IgG2a prevalence. Interestingly, CD8+ cell hypercellularity and lymphocyte immunosuppression were observed during the chronic phase (245 days post-infection) in mice infected by the most virulent clone. In the same way, the parasite-specific antibody response was more intense in P/209 cl1-infected mice over the acute phase. During the chronic phase this response remarkably dropped down in SO34 cl4-infected mice exclusively. Finally, P/209 cl1-infected mice presented a more severe inflammation and tissue damage in heart and quadriceps than SO34 cl4-infected mice. This comparative study showed differences between the two clones: a higher virulence in vivo being clearly associated with a greater ability to induce evasion mechanisms and severe tissue damage.

Type
Research Article
Copyright
© 2005 Cambridge University Press

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