Epidemiology and Infection



Salivary excretion of rabies virus by healthy vampire bats


A. AGUILAR-SETIEN a1c1, E. LOZA-RUBIO a2, M. SALAS-ROJAS a1, N. BRISSEAU a3, F. CLIQUET a3, P.-P. PASTORET a4, S. ROJAS-DOTOR a1, E. TESORO a5 and R. KRETSCHMER a1
a1 Unidad de Investigación Médica en Inmunología, Coordinación de Investigación Médica, Instituto Mexicano del Seguro Social, Centro Médico Nacional Siglo XXI, Hospital de Pediatría, México D.F., Mexico
a2 Centro Nacional de Investigaciones Disciplinarias en Microbiología, Instituto Nacional de Investigaciones Forestales y Agropecuarias, México D.F., Mexico
a3 Laboratoire d’études et de recherches sur la rage et la pathologie des animaux sauvages, Domaine de Pixérécourt, Malzeville, France
a4 Institute for Animal Health, Compton Newbury, Berks, UK
a5 Departamento de Investigación Experimental y Bioterio del Instituto Nacional de Ciencias Médicas y Nutrición ‘Salvador Zubirán’, México D.F., Mexico

Article author query
aguilar-setien a   [PubMed][Google Scholar] 
loza-rubio e   [PubMed][Google Scholar] 
salas-rojas m   [PubMed][Google Scholar] 
brisseau n   [PubMed][Google Scholar] 
cliquet f   [PubMed][Google Scholar] 
pastoret pp   [PubMed][Google Scholar] 
rojas-dotor s   [PubMed][Google Scholar] 
tesoro e   [PubMed][Google Scholar] 
kretschmer r   [PubMed][Google Scholar] 

Abstract

Salivary excretion of rabies virus was evaluated in 14 adult vampire bats (Desmodus rotundus) intramuscularly injected with a large dose (106 MICLD50) of vampire rabies virus variant CASS88. Saliva samples were obtained from surviving bats every other day for 30 days, then weekly for 2 months, and finally 1 and 2 years later. Rabies virus was isolated in murine neuroblastoma cells and in randomly selected cases by PCR. Rabies virus was not detected in the saliva of any of the 11 animals that succumbed (somewhat early) to rabies challenge, nor in the control bats. In contrast, virus was detected early, and only once (days 6, 6 and 21) in each of the three animals that survived rabies challenge and remained healthy for at least 2 years after challenge. At that time even vigorous dexamethasone and cyclosporine administration failed to provoke further viral excretion.

(Accepted July 22 2004)


Correspondence:
c1 Unidad de Investigación Médica en Inmunología, Coordinación de Investigación Médica, Instituto Mexicano del Seguro Social, Centro Médico Nacional Siglo XXI, Hospital de Pediatría, Av. Cuauhtemoc 330, Col. Doctores, 06725 México D.F., Mexico. (Email: varoaguila@prodigy.net.mx)


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