Parasitology



Detection of species-specific antibody response of humans and mice bitten by sand flies


I. ROHOUSOVA a1c1, S. OZENSOY a2, Y. OZBEL a2 and P. VOLF a1
a1 Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
a2 Department of Parasitology, Medical School and Hospital, Ege University, 35100 Bornova-Izmir, Turkey

Article author query
rohousova i   [PubMed][Google Scholar] 
ozensoy s   [PubMed][Google Scholar] 
ozbel y   [PubMed][Google Scholar] 
volf p   [PubMed][Google Scholar] 

Abstract

Sand fly saliva plays an important role in Leishmania transmission. We characterized the host antibody response to saliva from 3 sand fly species. Specific IgG was observed in sera from experimentally bitten mice as well as in sera from individuals living in the endemic area of Leishmania tropica in Sanliurfa, Turkey. Sera of Sanliurfa inhabitants showed high IgG levels against saliva of Phlebotomus sergenti and P. papatasi, the 2 most abundant sand fly species in this area, but did not react with saliva of the New World sand fly, Lutzomyia longipalpis. Patients with active Le. tropica lesions possessed significantly higher anti-P. sergenti IgG levels than the healthy individuals from the same place while anti-P. papatasi IgG levels were equal in both groups. Major protein bands in P. papatasi and P. sergenti saliva reacted with both, human and mice sera; in P. papatasi, however, mouse IgG recognized preferentially the 42 kDa protein band while the human IgG reacted strongly with the 30 kDa band. Our data suggest that the antibody response to sand fly saliva could be used for monitoring the exposure of humans and other hosts to sand flies and might be used as a marker of risks for Leishmania transmission in endemic areas.

(Received July 16 2004)
(Revised September 27 2004)
(Accepted September 28 2004)


Key Words: Phlebotomus; Lutzomyia; sand fly; salivary glands; saliva; IgG immune response; mouse; human.

Correspondence:
c1 Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic. Tel: +420 2 21951814. Fax: +420 2 24919704. E-mail: rohousova@seznam.cz


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