Cardiology in the Young



Original Article

Analysis of prothrombotic mutations and polymorphisms in children who developed thrombosis in the perioperative period of congenital cardiac surgery


Namik Özbek a1c1, F. Belgin Ataç a2, Selman V. Yildirim a1, Hasibe Verdi a2, Canan Yazici a3, Basak T. Yilmaz a1 and N. Kürsat Tokel a1
a1 Department of Pediatrics, Baskent University, Faculty of Medicine, Ankara, Turkey
a2 Department of Molecular Biology and Genetics, Baskent University, Faculty of Medicine, Ankara, Turkey
a3 Department of Medical Statistics, Baskent University, Faculty of Medicine, Ankara, Turkey

Article author query
ozbek n   [PubMed][Google Scholar] 
atac bf   [PubMed][Google Scholar] 
yildirim sv   [PubMed][Google Scholar] 
verdi h   [PubMed][Google Scholar] 
yazici c   [PubMed][Google Scholar] 
yilmaz bt   [PubMed][Google Scholar] 
tokel nk   [PubMed][Google Scholar] 

Abstract

In this study, we investigated some of the prothrombothic mutations and polymorphisms in 15 children with congenital cardiac malformations who developed severe thrombosis in the perioperative period following surgical repair. The mutations and polymorphisms included in the study were Factor V Leiden, prothrombin G20210A, methylentetrahydrofolate reductase C677T, endothelial nitric oxide synthase intron 4 VNTR, alpha-fibrinogen Thr312Ala, Factor XIII Val34Leu, and insertion or deletion of angiotensin 1 converting enzyme. Compared to the healthy Turkish subjects, our patients had a similar rate of mutation of Factor V Leiden, Factor XIII Val34Leu, and endothelial nitric oxide synthase a/b polymorphisms, but higher frequency of the prothrombotic angiotensin 1 converting enzyme deletion/deletion genotype, and lower frequency of the antithrombotic alpha fibrinogen Thr/Thr genotype. None of the patients exhibited mutations involving prothrombin G20210A or methylentetrahydrofolate reductase C677T. The results of our study suggest that, in addition to prothrombotic mutations such as Factor V Leiden, single-nucleotide polymorphisms should be considered in all children with congenital cardiac malformations who develop thrombosis.

Malformations of the heart are the most common of all serious lesions that are present at birth, with an incidence of 4 to 8 cases per 1,000 live births.1 If needed, corrective surgery is usually the optimal treatment for these anomalies, but perioperative morbidity and mortality still remain high due to several factors. Arterial or venous thrombosis, or both varieties of thrombosis, is among these factors. Prior to surgery, the most frequent time at which these children develop thrombosis is during cardiac catheterization. Postoperative thrombosis in this group of patients is a more complex disorder, which can affect both small and large vessels, and is associated with a high morbidity and mortality.

Recent studies indicate that both point mutations and single-nucleotide polymorphisms of genes that encode proteins involved in the coagulative and anticoagulative cascades are important risk factors for development of thrombosis. Patients with these risk factors are most likely to develop thrombosis when triggering elements, such as placement of catheters, prolonged immobilization, or surgery, are also present. In this study, we investigated some of the above-mentioned mutations and polymorphisms in children who developed thrombosis in the perioperative period after correction of congenital cardiac malformations.

(Accepted September 13 2004)


Key Words: Postoperative care; children; congenital heart malformations; point mutations.

Correspondence:
c1 Correspondence to: Namik Özbek MD, Baskent Üniversitesi Pediatri Bölümü, 6. Cadde, No: 72/3, 06490 Bahçelievler, Ankara, Turkey. Tel: +90 312 2130776/2234936; Fax: +90 312 2157597; E-mail: nozbek@tr.net


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