Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-18T00:56:26.129Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular markers to discriminate among four pest species of Helicoverpa (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  17 July 2008

G.T. Behere ,
W.T. Tay ,
D.A. Russell  and
P. Batterham
G.T. Behere*
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
W.T. Tay
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
D.A. Russell
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent, ME4 4TB, UK
P. Batterham
Affiliation:
Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio-21, Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville-3010, Australia
*
*Author for correspondence Fax: +61 (3)93475352 E-mail: g.behere@pgrad.unimelb.edu.au
Get access Rights & Permissions [Opens in a new window]

Abstract

The four significant pest species in the Helicoverpa genus (H. armigera, H. assulta, H. punctigera and H. zea) are morphologically similar and can only be reliably distinguished through dissection of adult genitalia. Two partial regions of the mitochondrial DNA (mtDNA), the cytochrome oxidase subunit I (COI) and the cytochrome b (Cyt b) genes were amplified by PCR and digested with restriction endonucleases. The restriction patterns, generated by the endonucleases BstZ17I and HphI, demonstrated reliable differentiation of the four Helicoverpa pest species. This technique is fast, reliable and effective at distinguishing specimens irrespective of their life stages and offers support to conventional taxonomic differentiation based on morphological characters.

Keywords

molecular species diagnosisPCR-RFLPmitochondrial DNAHelicoverpa
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 6 , December 2008 , pp. 599 - 603
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389–3402.CrossRefGoogle ScholarPubMed
Behere, G.T., Tay, W.T., Russell, D.A., Heckel, D.G., Appleton, B.R., Kranthi, K.R. & Batterham, P. (2007) Mitochondrial DNA analysis of field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) and of its relationship to H. zea. BMC Evolutionary Biology 7, 117.CrossRefGoogle ScholarPubMed
Daly, J.C. & Gregg, P. (1985) Genetic variation in Heliothis in Australia: species identification and gene flow in the two pest species H. armigera (Hübner) and H. punctigera (Wallengren) (Lepidoptera: Noctuidae). Bulletin of Entomological Research 75, 169–184.CrossRefGoogle Scholar
Greenstone, M.H., Stuart, M.K. & Haunerland, N.H. (1991) Using monoclonal antibodies for phylogenetic analysis: an example from the heliothinae (Lepidoptera: Noctuidae). Annals of Entomological Society of America 84, 457–464.CrossRefGoogle Scholar
Hardwick, D.F. (1965) The corn earworm complex. Memoirs of Entomological Society of Canada 40, 1–247.Google Scholar
Kranthi, S., Kranthi, K.R., Bharose, A.A. & Syed, S.N. (2005) A PCR-RFLP tool for differentiating Helicoverpa armigera and H. assulta (Lepidoptera: Noctuidae). Current Science 89, 1322–1323.Google Scholar
Matthews, M. (1999) Heliothine Moths of Australia: A Guide to Pest Bollworms and Related Noctuid Groups, Monographs on Australian Lepidoptera, vol. 7. pp. 105–119. Melbourne, Australia, CSIRO Publishing.Google Scholar
Ming, Q.-L. & Wang, C.-Z. (2006) Genetic differentiation of Helicoverpa armigera (Hübner) and H. assulta (Guenée) (Lepidoptera: Noctuidae) based on AFLP markers. Insect Science 13, 437–444.CrossRefGoogle Scholar
Mitter, C., Poole, R.W. & Matthews, M. (1993) Biosystematics of the Heliothinae (Lepidoptera: Noctuidae). Annual Review of Entomology 38, 207–225.CrossRefGoogle Scholar
Muraji, M. & Nakahara, S. (2002) Discrimination among pest species of Bactrocera (Diptera: Tephritidae) based on PCR-RFLP of the mitochondrial DNA. Applied Entomology and Zoology 37, 437–446.CrossRefGoogle Scholar
Pearce, S. (2003) The use of naturally occurring arthropod predators for the control of Helicoverpa spp. in grain crops in southeast Queensland. PhD thesis, The University of Queensland, Brisbane, Australia.Google Scholar
Pouge, M.A. (2004) New synonym of Helicoverpa zea (Boddie) and differentiation of adult males of H. zea and H. armigera (Hübner) (Lepidoptera: Noctuidae: Heliothinae). Annals of Entomological Society of America 97, 1222–1226.CrossRefGoogle Scholar
Seymour, P.R. (1978) Insects and other invertebrates intercepted in checks inspection of imported plant material in England and Wales during 1976 and 1977. Report from MAFF Plant Pathology Laboratory 10, 1–54.Google Scholar
Taylor, D.B. & Szalanski, A.L. (1999) Identification of Muscidifurax spp. by polymerase chain reaction-restriction fragment length polymorphism. Biological Control 15, 270–273.CrossRefGoogle Scholar
Trowell, S.C., Forrester, N.W., Garsia, K.A., Lang, G.A., Bird, L.J., Hill, A.S., Skerritt, J.H. & Daly, J. (2000) Rapid antibody-based field test to distinguish between Helicoverpa armigera (Lepidoptera: Noctuidae) and Helicoverpa punctigera (Lepidoptera: Noctuidae). Journal of Economic Entomology 93, 878–891.CrossRefGoogle ScholarPubMed
Venette, R.C., Davis, E.E., Zaspel, Z., Heisler, H. & Larson, M. (2003) Mini risk assessment, Old World bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). US Department of Agriculture, Animal and Plant Health Inspection Service. Available on-line: <http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/harmigerapra.pdf>(accessed 20 December 2007).(accessed+20+December+2007).>Google Scholar