Genetical Research



The IGF2-intron3-G3072A substitution explains a major imprinted QTL effect on backfat thickness in a Meishan×European white pig intercross


BART J. JUNGERIUS a1c1, ANNE-SOPHIE VAN LAERE a2, MARINUS F. W. TE PAS a3, BERNARD A. VAN OOST a4, LEIF ANDERSSON a2a5 and MARTIEN A. M. GROENEN a1
a1 Department of Animal Breeding and Genetics, Wageningen University, P.O. Box 40, 6700 AH Wageningen, The Netherlands
a2 Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Sweden
a3 Animal Sciences Group, Wageningen University and Research Centre, Lelystad, The Netherlands
a4 Department of Laboratory Animal Sciences, Utrecht University, Utrecht, The Netherlands
a5 Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden

Article author query
jungerius bj   [PubMed][Google Scholar] 
van laere as   [PubMed][Google Scholar] 
te pas mf   [PubMed][Google Scholar] 
van oost ba   [PubMed][Google Scholar] 
andersson l   [PubMed][Google Scholar] 
groenen ma   [PubMed][Google Scholar] 

Abstract

A paternally expressed QTL for muscle growth and backfat thickness (BFT) has previously been identified near the IGF2 locus on the distal tip of pig chromosome 2 (SSC2p) in three experimental F2 populations. Recently, a mutation in a regulatory element of the IGF2 gene was identified as the quantitative trait nucleotide (QTN) underlying the major QTL effect on muscle growth and BFT in crosses between Large White and Wild Boar or Pietrain. This study demonstrates that the IGF2 mutation also controls the paternally expressed QTL for backfat thickness in a cross between Meishan and European Whites. In addition, a comparison of QTL of backfat thickness measured by Hennessy grading probe (HGP) and by ultrasound measurement (USM) was made. In the USM analyses, the IFG2 mutation explains the entire QTL effect on SSC2p, whereas in the HGP analysis the presence of a second minor QTL can not be excluded. Finally, this study shows that this particular IGF2 mutation does not cause the paternally expressed QTL for teat number mapping to the same region of SSC2p as the BFT QTL.

(Received February 26 2004)
(Revised August 10 2004)


Correspondence:
c1 Tel: +31 317 484622. Fax: +31 317483929. e-mail: bart.jungerius@wur.nl


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