Parasitology



Molecular ecology of Schistosoma mansoni transmission inferred from the genetic composition of larval and adult infrapopulations within intermediate and definitive hosts


A. THERON a1c1, C. SIRE a1, A. ROGNON a1, F. PRUGNOLLE a2 and P. DURAND a2
a1 Parasitologie Fonctionnelle et Evolutive, UMR 5555 CNRS-UP, CBETM, Université, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France
a2 Centre d'Etude du Polymorphisme des Micro-organismes, Equipe ESS, UMR 9926 CNRS-IRD, 911 av. Agropolis, BP 5045, 34032 Montpellier, France

Article author query
theron a   [PubMed][Google Scholar] 
sire c   [PubMed][Google Scholar] 
rognon a   [PubMed][Google Scholar] 
prugnolle f   [PubMed][Google Scholar] 
durand p   [PubMed][Google Scholar] 

Abstract

We investigated the genotypic composition of the digenetic parasite Schistosoma mansoni for its adult stages within the definitive host (the wild rat, Rattus rattus) and for the larval stages within the intermediate host (the snail, Biomphalaria glabrata) both collected at the same transmission site. Our analyses are based upon the recognition and distribution of 200 different multilocus genotypes generated by RAPD markers. While intramolluscan larval infrapopulations are characterized by a low infection rate (0·6% on average) and low intra-host genetic diversity (1·1 genotype on average per infected snail), adult infrapopulations within rats showed a high infection rate (94%) and a substantial intra-host genetic diversity (34 genotypes on average) linked to high intensities (160 worms per host on average). A single definitive host bearing 105 different genotypes harboured 52% of the total genetic diversity detected within the whole parasite population. Analysis of the genetic data allowed the identification of various ecological, behavioural and immunological factors which are likely to enhance transmission of multiple parasite genotypes towards the vertebrate hosts. From the distribution of repeated identical multilocus genotypes within the parasite population and among the hosts, we have inferred different parameters of the cercarial transmission efficiency as well as patterns and processes by which vertebrate hosts acquire infection in the field.

(Received February 6 2004)
(Revised March 5 2004)
(Revised March 31 2004)
(Accepted March 31 2004)


Key Words: Schistosoma mansoni; Biomphalaria glabrata; Rattus rattus; genetic diversity; transmission; recruitment processes.

Correspondence:
c1 Parasitologie Fonctionnelle et Evolutive, UMR CNRS-UP 5555, CBETM, Université, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France. Tel: +33 4 68 66 2182. Fax: +33 4 68 66 2281. E-mail: theron@univ-perp.fr


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