Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts
Isolates of Cryptosporidium muris and C. serpentis were characterized from different hosts using nucleotide sequence analysis of the rDNA 18S and ITS1 regions, and the heat-shock (HSP-70) gene. Phylogenetic analysis confirmed preliminary evidence that C. muris is not a uniform species. Two distinct genotypes were identified within C. muris; (1) C. muris genotype A; comprising bovine and camel isolates of C. muris from different geographical locations, and (2) C. muris genotype B comprising C. muris isolates from mice, a hamster, a rock hyrax and a camel from the same enclosure. These 2 genotypes may represent separate species but further biological and molecular studies are required for confirmation.(Received September 13 1999)
(Revised November 11 1999)
(Accepted November 15 1999)
Key Words: Cryptosporidium muris; Cryptosporidium serpentis; 18S rDNA; ITS1; heat-shock gene; phylogenetic analysis.
c1 Corresponding author: Division of Veterinary and Biomedical Science, Murdoch University, Murdoch, WA, 6150, Australia. Tel: +08 9360 2457. Fax: +08 9310 4144. E-mail: firstname.lastname@example.org