Parasitology



Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts


U. M. MORGAN a1c1, L. XIAO a2, P. MONIS a3, I. SULAIMAN a2, I. PAVLASEK a4, B. BLAGBURN a5, M. OLSON a6, S. J. UPTON a7, N. V. KHRAMTSOV a7, A. LAL a2, A. ELLIOT a1 and R. C. A. THOMPSON a1
a1 World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, 6150, Australia
a2 Division of Parasitic Diseases, Centres for Disease Control and Prevention, Atlanta, Georgia, 30341, USA
a3 Microbiology Unit, Australian Water Quality Centre, Hodgson Road, Bolivar, SA, 5110, Australia
a4 State Veterinary Institute Prague, Pathology and Parasitology Department 165 03 Prague 6, Czech Republic
a5 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA
a6 University of Calgary, Calgary, Canada
a7 Division of Biology, Kansas State University, Manhattan, KS 66506, USA

Abstract

Isolates of Cryptosporidium muris and C. serpentis were characterized from different hosts using nucleotide sequence analysis of the rDNA 18S and ITS1 regions, and the heat-shock (HSP-70) gene. Phylogenetic analysis confirmed preliminary evidence that C. muris is not a uniform species. Two distinct genotypes were identified within C. muris; (1) C. muris genotype A; comprising bovine and camel isolates of C. muris from different geographical locations, and (2) C. muris genotype B comprising C. muris isolates from mice, a hamster, a rock hyrax and a camel from the same enclosure. These 2 genotypes may represent separate species but further biological and molecular studies are required for confirmation.

(Received September 13 1999)
(Revised November 11 1999)
(Accepted November 15 1999)


Key Words: Cryptosporidium muris; Cryptosporidium serpentis; 18S rDNA; ITS1; heat-shock gene; phylogenetic analysis.

Correspondence:
c1 Corresponding author: Division of Veterinary and Biomedical Science, Murdoch University, Murdoch, WA, 6150, Australia. Tel: +08 9360 2457. Fax: +08 9310 4144. E-mail: morgan@numbat.murdoch.edu.au


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