Whole genome genetic-typing in yeast using high-density oligonucleotide arrays

E. A. WINZELER a1c1, B. LEE , J. H. McCUSKER a2 and R. W. DAVIS 
a1 Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305–5307, USA
a2 Department of Microbiology, 3020, Duke University Medical Center, Durham, NC 27710, USA


Genome sequence information in combination with new technologies has allowed researchers to approach genetic problems in new ways. High-density oligonucleotide arrays were used to probe the genome content of the yeast Saccharomyces cerevisiae. We show that these arrays, containing oligonucleotides complementary to the sequenced strain of S. cerevisiae, can be used to identify open reading frames that are missing or present in higher or lower copy number in related isolates of S. cerevisiae. We apply this method to the characterization of the genome of a strain derived from a clinical isolate of S. cerevisiae. Our results show that the telomeres are the regions with the most variability between the two strains.

Key Words: oligonucleotide arrays; telomeres; yeast; hybridization.

c1 Author to whom correspondence should be addressed.