Zygote

Zygote (2004), 12:2:151-156 Cambridge University Press
Copyright © 2004 Cambridge University Press
doi:10.1017/S0967199404002722

Murine Asb-17 expression during mouse testis development and spermatogenesis


Kye-Seong Kim a1, Myung-Sun Kim a1, Soo-Kyung Kim a1 and Kwang-Hyun Baek a1c1
a1 Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea

Article author query
kim ks   [PubMed][Google Scholar] 
kim ms   [PubMed][Google Scholar] 
kim sk   [PubMed][Google Scholar] 
baek kh   [PubMed][Google Scholar] 

Abstract

In this study we isolated a murine mAsb-17 from mouse testis by RT-PCR using primers designed based on the sequences from the GenBank database. The sequence analysis showed that mAsb-17 encodes a 295 amino acid polypeptide with a molecular weight of approximately 34 kDa containing two ankyrin repeats and one SOCS box. The amino acid sequence of mASB-17 showed 87.5%, 98.3% and 92.9% identity with that of human, rat and dog, respectively. Interestingly, northern blot analysis showed that mAsb-17 was expressed only in the testis. The expression analysis by RT-PCR for mAsb-17 in mouse indicates that mAsb-17 is expressed from the fourth week after birth to adult, with the highest expression in round spermatids. Both northern blot and RT-PCR analyses suggest that mASB-17 may play essential roles in testis development and spermatogenesis.

(Received May 13 2003)
(Accepted June 19 2003)


Key Words: Ankyrin repeat; Asb-17; SOCS box; Spermatogenesis.

Correspondence:
c1 All correspondence to: Kwang-Hyun Baek, PhD, College of Medicine, Pochon CHA University, CHA General Hospital, 605 Yeoksam 1-dong, Kangnam-Gu, Seoul 135-081, Korea. Tel: +82 2 3468 3197. Fax: +82 2 3468 3264. e-mail: baek@cha.ac.kr


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