Parasitology



Mitochondrial genomic markers confirm the presence of the camel strain (G6 genotype) of Echinococcus granulosus in north-western China


L-H. ZHANG a1, J-J. CHAI a2, W. JIAO a2, Y. OSMAN a2 and D. P. McMANUS a1c1
a1 Molecular Parasitology Unit, Tropical Health Program, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, 300 Herston Road, Brisbane, Queensland 4029, Australia
a2 Institute of Endemic Disease Research and Control of Xinjiang Uygur Autonomous Region, 141 First Jianquan Road, Urumqi, Xinjiang 830002, People's Republic of China

Abstract

Twenty-eight isolates of E. granulosus, collected from humans at surgery, and a range of intermediate hosts, including sheep, cattle and camels from abattoirs in North and South Xinjiang Uygur Autonomous Region, People's Republic of China, were analysed for DNA sequence variation within regions of the mitochondrial cytochrome c oxidase I (COI) and NADH dehydrogenase subunit I (NDI) genes. The isolates were categorized into 2 distinct and uniform genotypic groupings, based on the sequences obtained, and the data clearly indicated that the camel/dog strain (G6 genotype) of E. granulosus as well as the cosmopolitan, common sheep strain (G1 genotype) occur in north Xinjiang. The presence of the camel strain has thus been confirmed in Xinjiang but it is evident from this and a previous molecular genetic survey of E. granulosus isolates from north-western China that the common sheep strain is the most predominant in the region. From the public health perspective, the majority of infected livestock will act as reservoirs of human infection there. During the course of the study, a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay, based on the NDI sequence variation, was developed that allows rapid discrimination of the G1 and G6 genotypes.

(Received May 2 1997)
(Revised June 18 1997)
(Accepted June 18 1997)


Key Words: Echinococcus granulosus; cystic hydatid disease; genotype; mitochondrial DNA; cytochrome c oxidase I (COI) gene; NADH dehydrogenase I (NDI) gene.

Correspondence:
c1 Corresponding author: Molecular Parasitology Unit, Tropical Health Program, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, 300 Herston Road, Brisbane, Queensland 4029, Australia. Tel: +61 7 3362 0401. Fax: +61 70 3362 0104. E-mail: donM@qimr.edu.au


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