Hostname: page-component-8448b6f56d-qsmjn Total loading time: 0 Render date: 2024-04-18T16:27:35.189Z Has data issue: false hasContentIssue false
  • English
  • Français

Differential expression of AP-1 transcription factor genes c-fos and c-jun in the helminth parasites Taenia crassiceps and Taenia solium

Published online by Cambridge University Press:  06 August 2004

J. MORALES-MONTOR ,
G. ESCOBEDO ,
M. RODRIGUEZ-DORANTES ,
N. TÉLLEZ-ASCENCIO ,
M. A. CERBÓN  and
C. LARRALDE
J. MORALES-MONTOR
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
G. ESCOBEDO
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
M. RODRIGUEZ-DORANTES
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
N. TÉLLEZ-ASCENCIO
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
M. A. CERBÓN
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
C. LARRALDE
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
Get access Rights & Permissions [Opens in a new window]

Abstract

Homologues of c-fos and c-jun from total DNA of Taenia crassiceps and Taenia solium were cloned and sequenced. The amino acid alignment analysis revealed that c-fos DNAs from T. crassiceps and T. solium were highly homologous (96%), and both have high homology compared to several mammalian c-fos proteins (93% to mouse, 96% to rat and 86% to human). The c-jun protein alignment showed higher homology (T. crassiceps and T. solium have 98%), when compared with mouse, rat and human, being 92%, 98% and 93% respectively. RT–PCR amplification of the parasite's total RNA, showed that T. crassiceps expressed both AP-1 complex genes, while T. solium only expressed c-fos. Southern blot hybridization analysis confirmed the true origin of each amplified gene. AP-1 transcription gene expression is regulated by oestradiol in the same fashion as their mammalian counterparts only in T. crassiceps. To study if AP-1 genes are involved in a physiological function of the cyst, reproduction was studied in vitro. Oestradiol treatment stimulated reproduction in T. crassiceps but not in T. solium cysticerci. This is the first report of the detection and functionality of AP-1 transcription factor genes in any species of helminth parasite.

Keywords

AP-1 transcription factorTaenia crassicepsTaenia soliumc-fos genec-jun gene
Type
Research Article
Information
Parasitology , Volume 129 , Issue 2 , August 2004 , pp. 233 - 243
Copyright
2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

ALEXANDER, J. & STIMSON, W. H. (1988). Sex hormones and course of parasitic infection. Parasitology Today 4, 189–193.CrossRefGoogle Scholar
BARNES, P. J. & ADCOCK, I. M. (1998). Transcription factors and asthma. European Respiratory Journal 12, 221–234.CrossRefGoogle Scholar
BOJALIL, R., TERRAZAS, L. I., GOVEZENSKY, T., SCIUTTO, E. & LARRALDE, C. (1993). Thymus-related cellular immune mechanisms in sex associated resistance to experimental murine cysticercosis (Taenia crassiceps). Journal of Parasitology 78, 384–389.CrossRefGoogle Scholar
CHINENOV, Y. & KERPPOLA, T. K. (2001). Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity. Oncogene 30, 2438–2452.CrossRefGoogle Scholar
CULBRETH, K. L., ESCH, G. W. & KUHN, R. E. (1972). Growth and development of larval Taenia crassiceps (Cestoda) II: the relationships between larval biomass and the uptake and incorporation of 14C-leucine. Experimental Parasitology 32, 272–281.CrossRefGoogle Scholar
FREEMAN, R. S. (1962). Studies on the biology of Taenia crassiceps (Zeder 1800) Rudolphi, 1810 (Cestoda). Canadian Journal of Zoology 40, 969–990.CrossRefGoogle Scholar
HUERTA, L., TERRAZAS, L. I., SCIUTTO, E., GOVEZENSKI, T. & LARRALDE, C. (1992). Immunological mediation of gonadal effects on experimental murine cysticercosis caused by Taenia crassiceps metacestodes. Journal of Parasitology 78, 471–476.CrossRefGoogle Scholar
HYDER, S. M., SHIPLEY, G. L. & STANCEL, G. M. (1995). Estrogen action in target cells: selective requirements for activation of different hormone response elements. Molecular and Cellular Endocrinology 112, 35–43.CrossRefGoogle Scholar
HYDER, S. M., STANCEL, G. M., NAWAZ, Z., McDONNELL, D. P. & LOOSE-MITCHELL, D. S. (1992). Identification of an estrogen response element in the 3′-flanking region of the murine c-fos protoonco gene. Journal of Biological Chemistry 25, 18047–18054.Google Scholar
KLEIN, S. L. (2000). The effects of hormones on sex differences in infection: from genes to behavior. Neurosciences and Biobehavioural Reviews 24, 627–638.CrossRefGoogle Scholar
KNOBIL, E., NEIL, J. D., EWING, L. L., GREENWALD, G. S., MARKERT, C. L. & PFA, D. W. (1988). The Physiology of Reproduction. Raven Press, New York.
KUSHNER, P. J., AGARD, D. A., GREENE, G. L., SCANLAN, T. S., SHIAU, A. K., UHT, R. M. & WEBB, P. (2000). Estrogen receptor pathways to AP-1. Journal of Steroid Biochemistry and Molecular Biology 30, 311–327.CrossRefGoogle Scholar
LARRALDE, C., MORALES, J., TERRAZAS, I., GOVEZENSKY, T. & ROMANO, M. C. (1995). Sex hormone changes induced by the parasite lead to feminization of the male host in murine Taenia crassiceps cysticercosis. Journal of Steroid Biochemistry and Molecular Biology 52, 575–580.CrossRefGoogle Scholar
LARRALDE, C., SOTELO, J., MONTOYA, R. M., PALENCIA, G., PADILLA, A., GOVEZENSKY, T., DIAZ, M. L. & SCIUTTO, E. (1990). Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium. Archives of Pathology and Laboratory Medicine 114, 926–928.Google Scholar
MAGGI, A. & PÉREZ, J. (1985). Role of female gonadal hormones in the CNS: clinical and experimental aspects. Life Sciences 37, 893–897.CrossRefGoogle Scholar
McLENNAN, D. A. & BROOKS, D. R. (1991). Parasites and sexual selection: a macroevolutionary perspective. Quality Reviews in Biology 66, 255–286.CrossRefGoogle Scholar
MORALES, J., VELASCO, T., TOVAR, V., FRAGOSO, G., FLEURY, A., BELTRAN, C., VILLALOBOS, N., ALUJA, A., RODARTE, L. F., SCIUTTO, E. & LARRALDE, C. (2002). Castration and pregnancy of rural pigs significantly increase the prevalence of naturally acquired Taenia solium cysticercosis. Veterinary Parasitology 80, 1–9.CrossRefGoogle Scholar
MORALES-MONTOR, J., BAIG, S., HALLAL-CALLEROS, C. & DAMIAN, R. T. (2002). Taenia crassiceps: androgen reconstitution of the host leads to protection during cysticercosis. Experimental Parasitology 4, 209–216.CrossRefGoogle Scholar
MORALES-MONTOR, J., BAIG, S., MITCHELL, R., DEWAY, K., HALLAL-CALLEROS, C. & DAMIAN, R. T. (2001). Immunoendocrine interactions during chronic cysticercosis determine male mouse feminization: role of IL-6. Journal of Immunology 167, 4527–4533.CrossRefGoogle Scholar
NEPHEW, K. P., WEBB, D. K., AKCALIK, C., MOULTON, B. C. & KHAN, S. A. (1993). Hormonal regulation and expression of the jun-C proto-oncogene in specific cell types of the rat uterus. Journal of Steroid Biochemistry and Molecular Biology 46, 281–287.CrossRefGoogle Scholar
PADILLA, A., GOVEZENSKY, T., SCIUTTO, E., JIMENEZ-GARCIA, L. F., GONSEBATT, M. E., RAMIREZ, P. & LARRALDE, C. (2001). Kinetics and characterization of cellular responses in the peritoneal cavity of mice infected with Taenia crassiceps. Journal of Parasitology 87, 591–599.CrossRefGoogle Scholar
RANSONE, L. J. & VERMA, I. M. (1990). Nuclear proto-oncogenes fos and jun. Annual Reviews in Cell Biology 6, 539–557.CrossRefGoogle Scholar
SCIUTTO, E., FRAGOSO, G., FLEURY, A., LACLETTE, J. P., SOTELO, J., ALUJA, A., VARGAS, L. & LARRALDE, C. (2000). Taenia solium disease in humans and pigs: an ancient parasitosis disease rooted in developing countries and emerging as a major health problem of global dimensions. Microbes and Infection 15, 1875–1890.CrossRefGoogle Scholar
SOTELO, J. & DEL BRUTO, O. H. (2000). Brain cysticercosis. Archives of Medical Research 31, 3–14.CrossRefGoogle Scholar
TERRAZAS, L. I., BOJALIL, R., GOVEZENSKY, T. & LARRALDE, C. (1994). A role for 17β-estradiol in immunoendocrine regulation of cysticercosis (Taenia crassiceps). Journal of Parasitology 80, 563–568.CrossRefGoogle Scholar
TERRAZAS, L. I., BOJALIL, R., GOVEZENSKY, T. & LARRALDE, C. (1998). Shift from an early protective TH1 immune response to a late permissive TH2 response in murine cysticercosis (Taenia crassiceps). Journal of Parasitology 84, 74–81.CrossRefGoogle Scholar
TOENJES, S. A., SPOLSKY, R. J., MOONEY, K. A. & KUHN, R. E. (1999). The systemic immune response of Balb/c mice infected with larval Taenia crassiceps is a mixed Th1/Th2-type response. Parasitology 118, 623–633.CrossRefGoogle Scholar
TOLEDO, A., FRAGOSO, G., ROSAS, G., HERNÁNDEZ, M., GEVORKIAN, G., LÓPEZ-CASILLAS, F., HERNÁNDEZ, B., ACERO, G., HUERTA, M., LARRALDE, C. & SCIUTTO, E. (2001). Two epitopes shared by Taenia crassiceps and Taenia solium confer protection against murine Taenia crassiceps cysticercosis along with a prominent Th1 response. Infection and Immunity 69, 1766–1773.CrossRefGoogle Scholar
WHITE, A. C. (1997). Neurocysticercosis: a major cause of neurological disease worldwide. Clinical Infectious Diseases 24, 101–113.CrossRefGoogle Scholar