Parasitology



Differential expression of AP-1 transcription factor genes c-fos and c-jun in the helminth parasites Taenia crassiceps and Taenia solium


J. MORALES-MONTOR a1c1, G. ESCOBEDO a1, M. RODRIGUEZ-DORANTES a2, N. TÉLLEZ-ASCENCIO a2, M. A. CERBÓN a2 and C. LARRALDE a1
a1 Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
a2 Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México

Article author query
morales-montor j   [PubMed][Google Scholar] 
escobedo g   [PubMed][Google Scholar] 
rodriguez-dorantes m   [PubMed][Google Scholar] 
tellez-ascencio n   [PubMed][Google Scholar] 
cerbon m   [PubMed][Google Scholar] 
larralde c   [PubMed][Google Scholar] 

Abstract

Homologues of c-fos and c-jun from total DNA of Taenia crassiceps and Taenia solium were cloned and sequenced. The amino acid alignment analysis revealed that c-fos DNAs from T. crassiceps and T. solium were highly homologous (96%), and both have high homology compared to several mammalian c-fos proteins (93% to mouse, 96% to rat and 86% to human). The c-jun protein alignment showed higher homology (T. crassiceps and T. solium have 98%), when compared with mouse, rat and human, being 92%, 98% and 93% respectively. RT–PCR amplification of the parasite's total RNA, showed that T. crassiceps expressed both AP-1 complex genes, while T. solium only expressed c-fos. Southern blot hybridization analysis confirmed the true origin of each amplified gene. AP-1 transcription gene expression is regulated by oestradiol in the same fashion as their mammalian counterparts only in T. crassiceps. To study if AP-1 genes are involved in a physiological function of the cyst, reproduction was studied in vitro. Oestradiol treatment stimulated reproduction in T. crassiceps but not in T. solium cysticerci. This is the first report of the detection and functionality of AP-1 transcription factor genes in any species of helminth parasite.

(Received October 30 2003)
(Revised January 8 2004)
(Revised January 22 2004)
(Accepted January 22 2004)


Key Words: AP-1 transcription factor; Taenia crassiceps; Taenia solium; c-fos gene; c-jun gene.

Correspondence:
c1 Departamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, AP 70228, México DF 04510, México. Tel: +5255 5622 3854. Fax: +5255 5622 3369. E-mail: jmontor66@biomedicas.unam.mx


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