A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues
J. P. DALTON a1 a2 , S. R. DAY a1 , A. C. DREW a1 and P. J. BRINDLEY a1
a1 Molecular Parasitology Unit, The Queensland Institute of Medical Research, The Bancroft Centre, Post Office, Royal Brisbane Hospital, Qld. 4029, and The Australian Centre for International and Tropical Health and Nutrition, Australia
a2 School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.(Received August 16 1996)
(Revised October 30 1996)
(Revised January 15 1997)
(Accepted January 15 1997)
Key Words: Schistosoma mansoni; Schistosoma japonicum; eggs; miracidia.
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