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Natural anti-proteases in rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis and the in vitro neutralization of fish α2-macroglobulin by the metalloprotease from the pathogenic haemoflagellate, Cryptobia salmositica

Published online by Cambridge University Press:  01 April 1997

X. ZUO  and
P. T. K. WOO
X. ZUO
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
P. T. K. WOO
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Abstract

Natural anti-proteases (α1-protease inhibitor (α1-PI; α1-antitrypsin) and α2-macroglobulin (α2-M)) were found in the blood of rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis. The α2-M inhibited Cryptobia salmositica proteases and was significantly higher in brook charr than in rainbow trout. Under in vitro conditions it took longer for the same number of parasites to neutralize the α2-M in charr than in trout blood. The haemolysis which occurred when C. salmositica was incubated in the blood of rainbow trout was due to neutralization of α2-M. This in vitro study also showed that it was the metalloprotease of C. salmositica that lysed red blood cells and the plasma of the two species of fishes initially prevented haemolysis by inhibiting the proteolytic activity. We suggest that the natural plasma α2-M plays an important role in defence against cryptobiosis in fishes.

Keywords

Cryptobia salmositicaOncorhynchus mykissSalvelinus fontinalisα2-macroglobulinα1-protease inhibitorprotease
Type
Research Article
Information
Parasitology , Volume 114 , Issue 4 , April 1997 , pp. 375 - 382
Copyright
© 1997 Cambridge University Press

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