British Journal of Nutrition

Full Papers

β-Cryptoxanthin from supplements or carotenoid-enhanced maize maintains liver vitamin A in Mongolian gerbils (Meriones unguiculatus) better than or equal to β-carotene supplements

Christopher Davisa1, Hua Jinga1, Julie A. Howea1a2, Torbert Rocheforda3 and Sherry A. Tanumihardjoa1 c1

a1 Department of Nutritional Sciences, University of Wisconsin-Madison, 1415 Linden Drive, Madison, WI 53706, USA

a2 Agronomy and Soils Department, Auburn University, Auburn, AL 36849, USA

a3 Department of Crop Sciences, University of Illinois-Urbana-Champaign, Urbana, IL 61801, USA

Abstract

Maize with enhanced provitamin A carotenoids (biofortified), accomplished through conventional plant breeding, maintains vitamin A (VA) status in Mongolian gerbils (Meriones unguiculatus). Two studies in gerbils compared the VA value of β-cryptoxanthin with β-carotene. Study 1 (n 47) examined oil supplements and study 2 (n 46) used maize with enhanced β-cryptoxanthin and β-carotene. After 4 weeks' depletion, seven or six gerbils were killed; remaining gerbils were placed into weight-matched groups of 10. In study 1, daily supplements were cottonseed oil, and 35, 35 or 17·5 nmol VA (retinyl acetate), β-cryptoxanthin or β-carotene, respectively, for 3 weeks. In study 2, one group of gerbils was fed a 50 % biofortified maize diet which contained 2·9 nmol β-cryptoxanthin and 3·2 nmol β-carotene/g feed. Other groups were given equivalent β-carotene or VA supplements based on prior-day intake from the biofortified maize or oil only for 4 weeks. In study 1, liver retinol was higher in the VA (0·74 (sd 0·11) μmol) and β-cryptoxanthin (0·65 (sd 0·10) μmol) groups than in the β-carotene (0·49 (sd 0·13) μmol) and control (0·41 (sd 0·16) μmol) groups (P < 0·05). In study 2, the VA (1·17 (sd 0·19) μmol) and maize (0·71 (sd 0·18) μmol) groups had higher liver retinol than the control (0·42 (sd 0·16) μmol) group (P < 0·05), whereas the β-carotene (0·57 (sd 0·21) μmol) group did not. Bioconversion factors (i.e. 2·74 μg β-cryptoxanthin and 2·4 μg β-carotene equivalents in maize to 1 μg retinol) were lower than the Institute of Medicine values.

(Received October 15 2007)

(Revised January 14 2008)

(Accepted January 16 2008)

(Online publication March 03 2008)

Correspondence:

c1 Corresponding author: Associate Professor Sherry A. Tanumihardjo, fax +1 608 262 860, email sherry@nutrisci.wisc.edu

Footnotes

Abbreviations: BW, body weight; IOM, Institute of Medicine; VA, vitamin A

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