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Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding

Published online by Cambridge University Press:  16 April 2004

S. A. ROBINSON ,
J. E. SMITH  and
P. A. MILLNER
S. A. ROBINSON
Affiliation:
School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
J. E. SMITH
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, UK
P. A. MILLNER
Affiliation:
School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
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Abstract

Previous studies have indicated that SAG1, the major surface molecule of the protozoan parasite Toxoplasma gondii, is an important attachment ligand for the host cell. However, the research data that supports this claim comes largely from studies investigating tachyzoite binding, and not SAG1 binding per se. In this study we successfully developed an in vitro attachment assay to directly evaluate the mechanism of SAG1-host cell binding. Competition experiments were then performed using SAG1 that had been pre-treated with the neoglycoprotein BSA-glucosamide or with antibody. Soluble BSA-glucosamide blocked SAG1 attachment to MDBK cells in a dose-dependent manner, implying that SAG1 binding is mediated, in part, via attachment to host cell surface glucosamine. Interestingly, pre-incubation of SAG1 in polyclonal sera from chronically infected mice failed to block binding. This challenges the assumption that anti-SAG1 antibodies block parasite attachment through the masking of SAG1 host cell binding domains. Taken together, this evidence presents new strategies for understanding SAG1-mediated attachment.

Keywords

Toxoplasma gondiiattachmentSAG1glucosamideantibody
Type
Research Article
Information
Parasitology , Volume 128 , Issue 4 , April 2004 , pp. 391 - 396
Copyright
© 2004 Cambridge University Press

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