Epidemiology and Infection



Short Paper

Genetic diversity of Streptococcus agalactiae strains colonizing the same pregnant woman


M. PÉREZ-RUIZ a1, J. M. RODRÍGUEZ-GRANGER a1, M. F. BAUTISTA-MARÍN a1, J. ROMERO-NOGUERA a1 and M. ROSA-FRAILE a1c1
a1 Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida Fuerzas Armadas, s/n 18014, Granada, Spain

Article author query
perez-ruiz m   [PubMed][Google Scholar] 
rodriguez-granger j   [PubMed][Google Scholar] 
bautista-marin m   [PubMed][Google Scholar] 
romero-noguera j   [PubMed][Google Scholar] 
rosa-fraile m   [PubMed][Google Scholar] 

Abstract

Pulsed-field gel electrophoresis (PFGE) of SmaI–DNA digests and serotyping was performed on 15 colonies of Streptococcus agalactiae (GBS) from each of 30 vaginal–rectal colonized women. Five distinct GBS serotypes were observed among the 30 specimens (Ia, Ib, II, III and V). In 29 of the 30 samples, the same serotype was observed among all 15 colonies; in the remaining specimen, the 15 colonies yielded two serotypes (II and V). The PFGE profiles of all colonies in 27 of the 30 subjects were indistinguishable within each subject. In the remaining women, different DNA profiles were identified among the colonies in each specimen, one of whom carried two different serotypes. Furthermore, strains of the same serotype belonging to different women were genetically heterogeneous.

(Accepted November 5 2003)


Correspondence:
c1 M. de la Rosa Fraile, Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida Fuerzas Armadas, s/n 18014, Granada, Spain.


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