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Identification of new chicken egg proteins by mass spectrometry-based proteomic analysis

Published online by Cambridge University Press:  13 June 2008

K. MANN ,
J.V. OLSEN ,
B. MAČEK ,
F. GNAD  and
M. MANN
K. MANN*
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduction, Am Klopferspitz 18, D-52182 Martinsried, Germany
J.V. OLSEN
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduction, Am Klopferspitz 18, D-52182 Martinsried, Germany
B. MAČEK
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduction, Am Klopferspitz 18, D-52182 Martinsried, Germany
F. GNAD
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduction, Am Klopferspitz 18, D-52182 Martinsried, Germany
M. MANN
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduction, Am Klopferspitz 18, D-52182 Martinsried, Germany
*
Corresponding author: mann@biochem.mpg.de
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Abstract

The chicken egg and foods containing egg components are an important part of human nutrition. Furthermore, eggs are a potential source of bioactive molecules and a potential delivery system for therapeutic proteins, explaining the continuing scientific interest in eggs and their components. Using mass spectrometry-based high-throughput proteomic techniques, 119 proteins in egg yolk, 78 proteins in egg white, and 528 proteins in the decalcified eggshell organic matrix have recently been identified. Most of these proteins were identified in their respective egg compartment for the first time. Some of these proteins were already known from chicken tissues or other egg compartments, but many were novel. In the eggshell soluble matrix 39 phosphoproteins containing more than 150 different phosphorylation sites were identified. Twenty-two of the identified phosphoproteins had not been recognized as phosphoproteins previously.

Keywords

chicken eggeggshellegg whiteegg yolkproteomicsemPAI: exponentially modified protein abundance indexMS: mass spectrometryOC: ovocleidinOCX: ovocalyxin
Type
Review Article
Information
World's Poultry Science Journal , Volume 64 , Issue 2 , June 2008 , pp. 209 - 218
Copyright
Copyright © World's Poultry Science Association 2008

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