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Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups

Published online by Cambridge University Press:  05 December 2003

I. MATEJUSOVÁ ,
M. GELNAR ,
O. VERNEAU ,
C. O. CUNNINGHAM  and
D. T. J. LITTLEWOOD
I. MATEJUSOVÁ
Affiliation:
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK
M. GELNAR
Affiliation:
Faculty of Sciences, Masaryk University, Kotlarska 2, 61135 Brno, Czech Republic
O. VERNEAU
Affiliation:
UMR 5555 CNRS, Parasitologie Fonctionnelle et Evolutive, CBETM, Université de Perpignan, A. Paul Alduy, 66860 Perpignan Cedex, France
C. O. CUNNINGHAM
Affiliation:
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK
D. T. J. LITTLEWOOD
Affiliation:
Parasitic Worms Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
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Abstract

Analyses of small subunit ribosomal RNA gene sequences of representatives of major taxa of Monopisthocotylea were performed to identify the sister group of Gyrodactylus. Nuclear ribosomal DNA sequencesNucleotide sequence data reported in this paper are available in the GenBank, DDBJ and EMBL databases under the accession numbers AJ566375-79, AJ566768, AJ567670-74, AJ581657. from the complete internal transcribed spacer (ITS) region were used to infer phylogeny of 37 Gyrodactylus species and Gyrodactyloides bychowskii, Macrogyrodactylus polypteri and Gyrdicotylus gallieni, using maximum likelihood, parsimony and Bayesian inference. The genus Gyrodactylus appeared to be a monophyletic group in all analyses, based on the present data set. Within the genus, there were 3 major groups recognized by high bootstrap values and posterior probabilities. None of the 6 subgenera appeared to be monophyletic, and the most basal subgenus G. (Gyrodactylus) was paraphyletic. Characteristics of the excretory system of Gyrodactylus do not seem to be conservative enough to reveal subgenera within Gyrodactylus and we suggest abandoning existing subgenera as indicators of phylogeny. The grouping of species based on the morphology of the ventral bar and marginal hooks seems to have sufficient power to infer relationships between the Gyrodactylus species.

Keywords

MonogeneaGyrodactylusSSUITSphylogeny
Type
Research Article
Information
Parasitology , Volume 127 , Issue 6 , December 2003 , pp. 603 - 611
Copyright
2003 Cambridge University Press

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