Parasitology

Original Articles

Extraction of microsporidial DNA from modified trichrome-stained clinical slides and subsequent species identification using PCR sequencing

K. S. CHANa1 c1 and T. H. KOHa1

a1 Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608

SUMMARY

Molecular techniques involving polymerase chain reaction (PCR) and sequencing provide a relatively simple and objective means of identifying microsporidia to species level. We modified previously described methods of DNA extraction and PCR conditions for identification of microsporidia from museum slides, clinical specimens and environmental samples and successfully identified Vittaforma corneae in 11 out of 13 cases of microsporidial infection from used trichrome-stained slides of corneal scrapings from HIV-negative patients with keratoconjunctivitis.

(Received November 27 2007)

(Revised February 02 2008)

(Revised February 14 2008)

(Accepted February 25 2008)

(Online publication April 28 2008)

Correspondence:

c1 Corresponding author. Tel: +65 91804878/+65 63214505. Fax: +65 6222 6826. E-mail address: chankiansing@yahoo.com.sg

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