Genetical Research

Research Article

Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method

Rumi Kondoa1 c1 p1, Etsuko T. Matsuuraa1 and Sadao I. Chigusaa1

a1 Department of Biology, Ohcanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112, Japan

Summary

By designing 3′ ends of primers in PCR (polymerase chain reaction), a specific DNA fragment was selectively amplified in the presence of a 103-fold excess of highly homologous (sequence difference ca. 2 %) opponent DNA. This technique was applied in detecting paternal leakage of mitochondrial DNA (mtDNA) in intraspecific crosses of Drosophila simulans and interspecific crosses of Drosophila simulans and Drosophila mauritiana. The mtDNA types of their progeny were analysed by selective amplification of the paternal mtDNA fragment possessing a polymorphic restriction site and detecting its cleaved fragments. Paternal mtDNA was detected in the progeny of 14 out of 16 crosses. The present result indicates small but frequent inheritance of sperm mtDNA in Drosophila, which is supportive to our previous finding.

Correspondence:

c1 Corresponding author.

p1 Present address: Department of Human Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411, Japan.

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