Parasitology

Original Articles

Specific tyrosine phosphorylation induced in Schistosoma mansoni miracidia by haemolymph from schistosome susceptible, but not resistant, Biomphalaria glabrata

A. J. WALKERa1 c1 and D. ROLLINSONa2

a1 School of Life Sciences, Kingston University, Penrhyn Road, Kingston upon Thames, Surrey KT1 2EE, UK

a2 Wolfson Wellcome Biomedical Laboratories, The Natural History Museum, Cromwell Road, London SW7 5BD, UK

SUMMARY

Molecular interplay during snail-schistosome interactions is poorly understood and there is much to discover concerning the effect of snail host molecules on molecular processes in schistosomes. Using the Biomphalaria glabrata – Schistosoma mansoni host-parasite system, the effects of exposure to haemolymph, derived from schistosome-resistant and susceptible snail strains, on protein tyrosine phosphorylation in miracidia have been investigated. Western blotting revealed several tyrosine phosphorylated proteins in this larval stage. Exposure of miracidia to haemolymph from susceptible snails for 60 min resulted in a striking, 5-fold, increase in the tyrosine phosphorylation of a 56 kDa (p56) S. mansoni protein. In contrast, haemolymph from resistant snails had little effect on protein tyrosine phosphorylation levels in miracidia. Confocal microscopy revealed that tyrosine phosphorylation was predominantly associated with proteins present in the tegument. Finally, treatment of miracidia with the tyrosine kinase inhibitor genistein significantly impaired their development into primary sporocysts. The results open avenues for research that focus on the potential importance of phospho-p56 to the outcome of schistosome infection in snails, and the significance of protein tyrosine kinase-mediated signalling events to the transformation of S. mansoni larvae.

(Received September 11 2007)

(Revised October 19 2007)

(Accepted October 20 2007)

(Online publication December 06 2007)

Correspondence:

c1 Corresponding author: School of Life Sciences, Kingston University, Kingston upon Thames, Surrey KT1 2EE, UK. Tel: +44 20 8547 2000. Fax: +44 20 8547 7497. E-mail: t.walker@kingston.ac.uk

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